Cited 10 times in
Linear Poly(ethylenimine) Cross-Linked by Methyl-β-Cyclodextrin for Gene Delivery.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 여영부 | - |
dc.contributor.author | 정한성 | - |
dc.date.accessioned | 2015-01-06T17:15:07Z | - |
dc.date.available | 2015-01-06T17:15:07Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1566-5232 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/99624 | - |
dc.description.abstract | Poly(ethylenimine) (PEI) is a cationic polymer extensively exploited for non-viral gene delivery; however, its wide application has been impeded by its cytotoxicity. PEI can assume either a branched or linear configuration. Whereas branched PEI (bPEI) is more chemically reactive and can form smaller complexes with DNA under salt-containing conditions, lPEI is generally less toxic and exhibits higher transfection efficiency. In this study, we cross-linked low-molecularweight lPEI with methyl β-cyclodextrin (MβCD) to form MβCD-lPEI (MLP). The structure of MLP was successfully characterized by NMR, FT-IR, MALDI-TOF and elemental analysis. In the standard serum-free transfection environment, MLP could effectively transfect glioblastoma, melanoma and hepatocarcinoma cells. A high transfection efficiency was maintained in the presence of serum. Apart from its high transfection efficiency, MLP was found to have negligible cytotoxicity over a wide range of concentrations and to exhibit a low membrane disruptive capacity ex vivo. MLP warrants further development as a promising gene delivery system for future research. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 258~268 | - |
dc.relation.isPartOf | CURRENT GENE THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Chemistry Techniques, Synthetic | - |
dc.subject.MESH | Cross-Linking Reagents/chemistry | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Transfer Techniques* | - |
dc.subject.MESH | Genetic Therapy/methods | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Magnetic Resonance Spectroscopy | - |
dc.subject.MESH | Mice, Inbred C57BL | - |
dc.subject.MESH | Molecular Structure | - |
dc.subject.MESH | Molecular Weight | - |
dc.subject.MESH | Neoplasms/genetics | - |
dc.subject.MESH | Neoplasms/therapy | - |
dc.subject.MESH | Polyethyleneimine/chemistry* | - |
dc.subject.MESH | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.subject.MESH | Spectroscopy, Fourier Transform Infrared | - |
dc.subject.MESH | Transfection/methods | - |
dc.subject.MESH | beta-Cyclodextrins/chemistry* | - |
dc.title | Linear Poly(ethylenimine) Cross-Linked by Methyl-β-Cyclodextrin for Gene Delivery. | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Wing-Fu Lai | - |
dc.contributor.googleauthor | David W. Green | - |
dc.contributor.googleauthor | Han-Sung Jung | - |
dc.identifier.doi | 10.2174/1566523214666140612160042 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02347 | - |
dc.contributor.localId | A03758 | - |
dc.relation.journalcode | J00666 | - |
dc.identifier.eissn | 1875-5631 | - |
dc.identifier.pmid | 25039611 | - |
dc.identifier.url | http://www.eurekaselect.com/122715/article | - |
dc.subject.keyword | Cancer | - |
dc.subject.keyword | cyclodextrin | - |
dc.subject.keyword | gene delivery | - |
dc.subject.keyword | gene therapy | - |
dc.subject.keyword | non-viral gene vector | - |
dc.subject.keyword | poly (ethylenimine) | - |
dc.contributor.alternativeName | Lai, Wing Fu | - |
dc.contributor.alternativeName | Jung, Han Sung | - |
dc.contributor.affiliatedAuthor | Lai, Wing Fu | - |
dc.contributor.affiliatedAuthor | Jung, Han Sung | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 14 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 258 | - |
dc.citation.endPage | 268 | - |
dc.identifier.bibliographicCitation | CURRENT GENE THERAPY, Vol.14(4) : 258-268, 2014 | - |
dc.identifier.rimsid | 38961 | - |
dc.type.rims | ART | - |
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