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Cited 16 times in

Linear Poly(ethylenimine) Cross-Linked by Methyl-β-Cyclodextrin for Gene Delivery.

DC Field Value Language
dc.contributor.author여영부-
dc.contributor.author정한성-
dc.date.accessioned2015-01-06T17:15:07Z-
dc.date.available2015-01-06T17:15:07Z-
dc.date.issued2014-
dc.identifier.issn1566-5232-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/99624-
dc.description.abstractPoly(ethylenimine) (PEI) is a cationic polymer extensively exploited for non-viral gene delivery; however, its wide application has been impeded by its cytotoxicity. PEI can assume either a branched or linear configuration. Whereas branched PEI (bPEI) is more chemically reactive and can form smaller complexes with DNA under salt-containing conditions, lPEI is generally less toxic and exhibits higher transfection efficiency. In this study, we cross-linked low-molecularweight lPEI with methyl β-cyclodextrin (MβCD) to form MβCD-lPEI (MLP). The structure of MLP was successfully characterized by NMR, FT-IR, MALDI-TOF and elemental analysis. In the standard serum-free transfection environment, MLP could effectively transfect glioblastoma, melanoma and hepatocarcinoma cells. A high transfection efficiency was maintained in the presence of serum. Apart from its high transfection efficiency, MLP was found to have negligible cytotoxicity over a wide range of concentrations and to exhibit a low membrane disruptive capacity ex vivo. MLP warrants further development as a promising gene delivery system for future research.-
dc.description.statementOfResponsibilityopen-
dc.format.extent258~268-
dc.relation.isPartOfCURRENT GENE THERAPY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHChemistry Techniques, Synthetic-
dc.subject.MESHCross-Linking Reagents/chemistry-
dc.subject.MESHFemale-
dc.subject.MESHGene Transfer Techniques*-
dc.subject.MESHGenetic Therapy/methods-
dc.subject.MESHHumans-
dc.subject.MESHMagnetic Resonance Spectroscopy-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHMolecular Structure-
dc.subject.MESHMolecular Weight-
dc.subject.MESHNeoplasms/genetics-
dc.subject.MESHNeoplasms/therapy-
dc.subject.MESHPolyethyleneimine/chemistry*-
dc.subject.MESHSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization-
dc.subject.MESHSpectroscopy, Fourier Transform Infrared-
dc.subject.MESHTransfection/methods-
dc.subject.MESHbeta-Cyclodextrins/chemistry*-
dc.titleLinear Poly(ethylenimine) Cross-Linked by Methyl-β-Cyclodextrin for Gene Delivery.-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Oral Biology (구강생물학)-
dc.contributor.googleauthorWing-Fu Lai-
dc.contributor.googleauthorDavid W. Green-
dc.contributor.googleauthorHan-Sung Jung-
dc.identifier.doi10.2174/1566523214666140612160042-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02347-
dc.contributor.localIdA03758-
dc.relation.journalcodeJ00666-
dc.identifier.eissn1875-5631-
dc.identifier.pmid25039611-
dc.identifier.urlhttp://www.eurekaselect.com/122715/article-
dc.subject.keywordCancer-
dc.subject.keywordcyclodextrin-
dc.subject.keywordgene delivery-
dc.subject.keywordgene therapy-
dc.subject.keywordnon-viral gene vector-
dc.subject.keywordpoly (ethylenimine)-
dc.contributor.alternativeNameLai, Wing Fu-
dc.contributor.alternativeNameJung, Han Sung-
dc.contributor.affiliatedAuthorLai, Wing Fu-
dc.contributor.affiliatedAuthorJung, Han Sung-
dc.rights.accessRightsfree-
dc.citation.volume14-
dc.citation.number4-
dc.citation.startPage258-
dc.citation.endPage268-
dc.identifier.bibliographicCitationCURRENT GENE THERAPY, Vol.14(4) : 258-268, 2014-
dc.identifier.rimsid38961-
dc.type.rimsART-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers

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