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Failure of Tooth Formation Mediated by miR-135a Overexpression via BMP Signaling

Authors
 E.J. Kim  ;  M.J. Lee  ;  L. Li  ;  K.S. Yoon  ;  K.S. Kim  ;  H.S. Jung 
Citation
 JOURNAL OF DENTAL RESEARCH, Vol.93(6) : 571-575, 2014 
Journal Title
 JOURNAL OF DENTAL RESEARCH 
ISSN
 0022-0345 
Issue Date
2014
MeSH
Animals ; Bone Morphogenetic Protein 2/genetics ; Bone Morphogenetic Protein 2/pharmacology ; Bone Morphogenetic Protein Receptors, Type I/genetics* ; Epithelium/metabolism ; Gene Expression Regulation/genetics* ; Genetic Vectors/genetics ; Lentivirus/genetics ; Male ; Mesoderm/metabolism ; Mice ; Mice, Nude ; MicroRNAs/antagonists & inhibitors ; MicroRNAs/genetics* ; Odontogenesis/genetics* ; Recombinant Proteins/pharmacology ; Signal Transduction/genetics* ; Tooth Germ/drug effects ; Tooth Germ/metabolism ; Tooth Germ/transplantation ; Transcription, Genetic/genetics ; Transforming Growth Factor beta/pharmacology ; Substances
Keywords
Bmp receptors ; lentivirus ; miRNA expression ; miRNA inhibitor ; microRNA ; tooth development
Abstract
MicroRNAs (miRNAs) are known to regulate a variety of gene functions in many tissues and organs, but their expression and function in tooth development are not well-understood. A specific miRNA, miR-135a, was determined to be highly expressed at the bud stage. Interestingly, after the cap stage, miR-135a was expressed in the epithelium and mesenchyme but not in the inner enamel epithelium. To identify the relationship between miR-135a and its putative target genes, Bmpr-Ia and Bmpr-Ib, in early tooth development, miR-135a was ectopically overexpressed with a lentivirus. This overexpression resulted in the repression of Bmpr-Ia and Bmpr-Ib. Furthermore, miR-135a inhibited both Bmpr-Ia and Bmpr-Ib transcription. BMP2 proteins were expressed ectopically in tooth germs during the cap stage to determine the relationship between miR-135a and BMP signaling in early tooth development. When miR-135a was ectopically expressed, no tooth formation was observed after 4 wk of incubation in the kidney capsule. This study suggested that Bmp signaling, specifically Bmpr-Ia and Bmpr-Ib, regulates tooth formation via miR-135a.
Full Text
http://jdr.sagepub.com/content/93/6/571.long
DOI
10.1177/0022034514529303
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun-Jung(김은정) ORCID logo https://orcid.org/0000-0002-9515-7590
Yoon, Kyung Sik(윤경식)
Lee, Min Jung(이민정)
Li, Liwen(이여문)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98719
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