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실험적 당뇨 흰쥐에서 사구체의 크기에 따른 유전자 발현의 차이

Other Titles
 Differential Gene Expression According to the Size ofGlomeruli in Experimental Diabetic Nephropathy 
 곽승재  ;  정동섭  ;  강신욱  ;  한대석  ;  문성진  ;  이정은  ;  김동기  ;  이태희  ;  한승혁  ;  이금희  ;  김진주 
 Korean Journal of Nephrology, Vol.26(2) : 137-154, 2007 
Journal Title
 Korean Journal of Nephrology 
Issue Date
Purpose: Although a few gene-profiling studies with whole renal tissue have been described in experimental diabetic nephropathy, there is only one microarray study using diabetic glomeruli. Furthermore, hypertrophic glomeruli have not been explored. The purpose of this study is to elucidate gene expression profiles of hypertrophic glomeruli in early diabetic nephropathy. Methods: Forty-male Sprague-Dawley rats were injected with diluent (N=20) or streptozotocin intraperitoneally (DM, N=20) and were sacrificed at 6- and 12-week. Glomeruli were isolated by sieving technique. Glomeruli from 125 and 75 m sieves were classified into large (hypertrophic, DM-LG) and small glomeruli (DM-SG), respectively. After RNA extraction, hybridization was performed on the Rat cDNA 5K chip in triplicate, and slides were analyzed. The significant genes were selected using significant analysis of microarray. Results: At 6-week, hierarchical clustering revealed that gene expression profiles of DM-LG were different from those of DM-SG, whereas DM-SG and C glomeruli showed similar gene expression pattern. In contrast, gene expression profiles at 12-week were similar between DM-LG and DM-SG, whereas C glomeruli showed different gene expression pattern from DM glomeruli. At 6-week, a total of 207 genes showed greater than 1.5-fold differential expression. 149 genes were upregulated, whereas 58 were downregulated in DM-LG. On the other hand, differential gene expression greater than 1.4 - fold was observed in 37 genes at 12-week, upregulated in 26 and downregulated in 11. Conclusion: These results suggest that the gene expression profiles of DM-LG are different from DM-SG, and the gene expression patterns change with the progression of diabetic nephropathy.
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1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Shin Wook(강신욱) ORCID logo https://orcid.org/0000-0002-5677-4756
Kwak, Seung Jae(곽승재)
Kim, Dong Ki(김동기)
Kim, Jin Ju(김진주)
Moon, Sung Jin(문성진)
Li, Jin Ji(이금희)
Lee, Jung Eun(이정은) ORCID logo https://orcid.org/0000-0003-0917-2872
Lee, Tae Hee(이태희)
Jung, Dong Sub(정동섭)
Han, Dae Suk(한대석)
Han, Seung Hyeok(한승혁) ORCID logo https://orcid.org/0000-0001-7923-5635
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