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실험적 당뇨 흰쥐에서 사구체의 크기에 따른 유전자 발현의 차이
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김진주 | - |
dc.contributor.author | 문성진 | - |
dc.contributor.author | 강신욱 | - |
dc.contributor.author | 곽승재 | - |
dc.contributor.author | 김동기 | - |
dc.contributor.author | 이금희 | - |
dc.contributor.author | 이정은 | - |
dc.contributor.author | 이태희 | - |
dc.contributor.author | 정동섭 | - |
dc.contributor.author | 한대석 | - |
dc.contributor.author | 한승혁 | - |
dc.date.accessioned | 2014-12-21T17:06:54Z | - |
dc.date.available | 2014-12-21T17:06:54Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1225-0015 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96973 | - |
dc.description.abstract | Purpose: Although a few gene-profiling studies with whole renal tissue have been described in experimental diabetic nephropathy, there is only one microarray study using diabetic glomeruli. Furthermore, hypertrophic glomeruli have not been explored. The purpose of this study is to elucidate gene expression profiles of hypertrophic glomeruli in early diabetic nephropathy. Methods: Forty-male Sprague-Dawley rats were injected with diluent (N=20) or streptozotocin intraperitoneally (DM, N=20) and were sacrificed at 6- and 12-week. Glomeruli were isolated by sieving technique. Glomeruli from 125 and 75 m sieves were classified into large (hypertrophic, DM-LG) and small glomeruli (DM-SG), respectively. After RNA extraction, hybridization was performed on the Rat cDNA 5K chip in triplicate, and slides were analyzed. The significant genes were selected using significant analysis of microarray. Results: At 6-week, hierarchical clustering revealed that gene expression profiles of DM-LG were different from those of DM-SG, whereas DM-SG and C glomeruli showed similar gene expression pattern. In contrast, gene expression profiles at 12-week were similar between DM-LG and DM-SG, whereas C glomeruli showed different gene expression pattern from DM glomeruli. At 6-week, a total of 207 genes showed greater than 1.5-fold differential expression. 149 genes were upregulated, whereas 58 were downregulated in DM-LG. On the other hand, differential gene expression greater than 1.4 - fold was observed in 37 genes at 12-week, upregulated in 26 and downregulated in 11. Conclusion: These results suggest that the gene expression profiles of DM-LG are different from DM-SG, and the gene expression patterns change with the progression of diabetic nephropathy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 137~154 | - |
dc.relation.isPartOf | Korean Journal of Nephrology | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | 실험적 당뇨 흰쥐에서 사구체의 크기에 따른 유전자 발현의 차이 | - |
dc.title.alternative | Differential Gene Expression According to the Size ofGlomeruli in Experimental Diabetic Nephropathy | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | 곽승재 | - |
dc.contributor.googleauthor | 정동섭 | - |
dc.contributor.googleauthor | 강신욱 | - |
dc.contributor.googleauthor | 한대석 | - |
dc.contributor.googleauthor | 문성진 | - |
dc.contributor.googleauthor | 이정은 | - |
dc.contributor.googleauthor | 김동기 | - |
dc.contributor.googleauthor | 이태희 | - |
dc.contributor.googleauthor | 한승혁 | - |
dc.contributor.googleauthor | 이금희 | - |
dc.contributor.googleauthor | 김진주 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01029 | - |
dc.contributor.localId | A01364 | - |
dc.contributor.localId | A00053 | - |
dc.contributor.localId | A00170 | - |
dc.contributor.localId | A02692 | - |
dc.contributor.localId | A03266 | - |
dc.contributor.localId | A03594 | - |
dc.contributor.localId | A04272 | - |
dc.contributor.localId | A04304 | - |
dc.contributor.localId | A03119 | - |
dc.contributor.localId | A00400 | - |
dc.relation.journalcode | J02065 | - |
dc.contributor.alternativeName | Kim, Jin Ju | - |
dc.contributor.alternativeName | Moon, Sung Jin | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.alternativeName | Kwak, Seung Jae | - |
dc.contributor.alternativeName | Kim, Dong Ki | - |
dc.contributor.alternativeName | Li, Jin Ji | - |
dc.contributor.alternativeName | Lee, Jung Eun | - |
dc.contributor.alternativeName | Lee, Tae Hee | - |
dc.contributor.alternativeName | Jung, Dong Sub | - |
dc.contributor.alternativeName | Han, Dae Suk | - |
dc.contributor.alternativeName | Han, Seung Hyeok | - |
dc.contributor.affiliatedAuthor | Kim, Jin Ju | - |
dc.contributor.affiliatedAuthor | Moon, Sung Jin | - |
dc.contributor.affiliatedAuthor | Kang, Shin Wook | - |
dc.contributor.affiliatedAuthor | Kwak, Seung Jae | - |
dc.contributor.affiliatedAuthor | Li, Jin Ji | - |
dc.contributor.affiliatedAuthor | Lee, Tae Hee | - |
dc.contributor.affiliatedAuthor | Jung, Dong Sub | - |
dc.contributor.affiliatedAuthor | Han, Dae Suk | - |
dc.contributor.affiliatedAuthor | Han, Seung Hyeok | - |
dc.contributor.affiliatedAuthor | Lee, Jung Eun | - |
dc.contributor.affiliatedAuthor | Kim, Dong Ki | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 26 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 137 | - |
dc.citation.endPage | 154 | - |
dc.identifier.bibliographicCitation | Korean Journal of Nephrology, Vol.26(2) : 137-154, 2007 | - |
dc.identifier.rimsid | 37688 | - |
dc.type.rims | ART | - |
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