11 389

Cited 89 times in

Activation of the renin–angiotensin system within podocytes in diabetes

 T-H Yoo  ;  J-J Li  ;  S-W Kang  ;  D S Han  ;  J E Lee  ;  S H Han  ;  H J Kim  ;  J S Kim  ;  H Y Choi  ;  D-R Ryu  ;  S-J Kwak  ;  D-S Jung  ;  J-J Kim 
 KIDNEY INTERNATIONAL, Vol.71(10) : 1019-1027, 2007 
Journal Title
Issue Date
The autocrine and paracrine activation of the renin-angiotensin system (RAS) within cells of the kidney plays a role in the overall pathophysiology of the renal disease due to diabetes. In this study, we focus on components of the RAS in the podocyte as these cells are important in the pathogenesis of glomerulosclerosis and proteinuria. Immortalized mouse podocytes were exposed to media containing normal glucose (NG) or high glucose (HG) for in vitro studies. In vivo studies utilized kidney tissue obtained from rats treated for 3 months with streptozotocin to induce diabetes. Angiotensinogen (AGT) and the angiotensin II (AII) type 1 receptor mRNA and protein were significantly increased in the podocytes cultured under the high glucose conditions. Both angiotensins I and II levels were significantly higher in cell lysates and the conditioned media of cells grown in high glucose. There were no differences in renin activity, angiotensin-converting enzyme level, or AII type 2 receptor level. Glomerular AGT and AII type 1 receptor assessed by means of immunohistochemistry were increased in diabetic rats compared with the control rats. Other measured components of the RAS within the glomeruli were not different. We suggest that increased AGT, an attendant increase in AII and increased AII type 1 receptor in podocytes experiencing diabetic conditions play an important role in the pathogenesis of diabetic nephropathy.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
Yonsei Authors
Kang, Shin Wook(강신욱) ORCID logo https://orcid.org/0000-0002-5677-4756
Kwak, Seung Jae(곽승재)
Kim, Joo Sung(김주성)
Kim, Jin Ju(김진주)
Kim, Hyung Jong(김형종)
Yoo, Tae Hyun(유태현) ORCID logo https://orcid.org/0000-0002-9183-4507
Li, Jin Ji(이금희)
Lee, Jung Eun(이정은) ORCID logo https://orcid.org/0000-0003-0917-2872
Jung, Dong Sub(정동섭)
Choi, Hoon Young(최훈영) ORCID logo https://orcid.org/0000-0002-4245-0339
Han, Dae Suk(한대석)
Han, Seung Hyeok(한승혁) ORCID logo https://orcid.org/0000-0001-7923-5635
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.