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Whole genome analysis for liver metastasis gene signatures in colorectal cancer

 Dong Hyuk Ki  ;  Hei-Cheul Jeung  ;  Sun Young Rha  ;  Hyun Chul Chung  ;  Nam Kyu Kim  ;  Won Suk Lee  ;  Gui Youn Lee  ;  Seung Hee Kang  ;  Chan Hee Park 
 INTERNATIONAL JOURNAL OF CANCER, Vol.121(9) : 2005-2012, 2007 
Journal Title
Issue Date
Adult ; Aged ; Aged, 80 and over ; Colorectal Neoplasms/genetics* ; Colorectal Neoplasms/pathology* ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic* ; Genome, Human/genetics* ; Humans ; Liver Neoplasms/genetics* ; Liver Neoplasms/secondary* ; Male ; Middle Aged ; Oligonucleotide Array Sequence Analysis ; Organ Specificity ; Polymerase Chain Reaction ; RNA, Messenger/genetics
colorectal cancer ; liver metastasis ; gene expression profiling ; cDNA microarray
Liver metastasis is one of the major causes of death in colorectal cancer (CRC) patients. To understand this process, we investigated whether the gene expression profiling of matched colorectal carcinomas and liver metastases could reveal key molecular events involved in tumor progression and metastasis. We performed experiments using a cDNA microarray containing 17,104 genes with the following tissue samples: paired tissues of 25 normal colorectal mucosa, 27 primary colorectal tumors, 13 normal liver and 27 liver metastasis, and 20 primary colorectal tumors without liver metastasis. To remove the effect of normal cell contamination, we selected 4,583 organ-specific genes with a false discovery rate (FDR) of 0.0067% by comparing normal colon and liver tissues using significant analysis of microarray, and these genes were excluded from further analysis. We then identified and validated 46 liver metastasis-specific genes with an accuracy of 83.3% by comparing the expression of paired primary colorectal tumors and liver metastases using prediction analysis of microarray. The 46 selected genes contained several known oncogenes and 2 ESTs. To confirm that the results correlated with the microarray expression patterns, we performed RT-PCR with WNT5A and carbonic anhydrase II. Additionally, we observed that 21 of the 46 genes were differentially expressed (FDR = 2.27%) in primary tumors with synchronous liver metastasis compared with primary tumors without liver metastasis. We scanned the human genome using a cDNA microarray and identified 46 genes that may play an important role in the progression of liver metastasis in CRC.
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1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Cancer Metastasis Research Center (암전이연구센터) > 1. Journal Papers
Yonsei Authors
Ki, Dong Hyuk(기동혁)
Kim, Nam Kyu(김남규) ORCID logo https://orcid.org/0000-0003-0639-5632
Rha, Sun Young(라선영) ORCID logo https://orcid.org/0000-0002-2512-4531
Park, Chan Hee(박찬희)
Lee, Gui Youn(이귀연)
Lee, Won Suk(이원석)
Chung, Hyun Cheol(정현철) ORCID logo https://orcid.org/0000-0002-0920-9471
Jeung, Hei Cheul(정희철) ORCID logo https://orcid.org/0000-0003-0952-3679
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