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사람중이점막세포에서 Uridine-5’-Triphosphate 자극에 의한 Cystic Fibrosis Transmembrane Conductance Regulator와 Ca2+-Activated Chloride Channel을 통한 Chloride 분비 활성화

Other Titles
 Uridine-5’-Triphosphate Stimulates Chloride Secretion via Cystic Fibrosis Transmembrane Conductance Regulator and Ca2+-Activated Chloride Channels in Cultured Human Middle Ear Epithelial Cells 
 Eun Jin Son  ;  Heon Young Gee  ;  Min-Goo Lee  ;  Won-Sang Lee  ;  Jae Young Choi 
 Korean Journal of Otorhinolaryngology-Head and Neck Surgery, Vol.54(12) : 840-846, 2011 
Journal Title
Korean Journal of Otorhinolaryngology-Head and Neck Surgery
Issue Date
UTP ; Chloride ; Ion channel ; Middle ear
Background and ObjectivesZZNucleotide binding to purinergic P2Y receptors contributes to the regulation of fluid and ion transport in the middle ear epithelial cells. Here, we investigated
the regulatory mechanism of the P2Y2 receptor agonist, uridine-5’-triphosphate (UTP), on Cl- transport in cultured normal human middle ear epithelial (NHMEE) cells.
Materials and MethodZZElectrophysiological measurements were performed in monolayers of cultured NHMEE cells. Short circuit currents (Isc) were measured from the cells mounted in Ussing chambers under various conditions.
ResultsZZApical addition of UTP in presence of amiloride evoked a transient rise and a sustained
response in Isc due to Cl- efflux. Application of different Cl- channel blockers to the apical
side of the cells significantly decreased UTP-induced Isc. Niflumic acid (NFA), a known blocker of Ca2+-activated chloride channels (CACC), and CFTRinh172, a selective inhibitor of cystic fibrosis transmembrane conductance regulator (CFTR), partially inhibited the UTP-induced
Cl- secretion, respectively.
ConclusionZZCl- transport across the airway epithelia plays a predominant role in regulating
airway hydration. In this study, UTP is shown to increase both CACC and CFTR-dependent
Cl- secretion in NHMEE cells, suggesting their role in fluid and ion transport in the middle ear epithelium
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1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers
Yonsei Authors
Son, Eun Jin(손은진)
Lee, Min Goo(이민구) ORCID logo https://orcid.org/0000-0001-7436-012X
Lee, Won Sang(이원상)
Gee, Heon Yung(지헌영) ORCID logo https://orcid.org/0000-0002-8741-6177
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
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