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사람중이점막세포에서 Uridine-5’-Triphosphate 자극에 의한 Cystic Fibrosis Transmembrane Conductance Regulator와 Ca2+-Activated Chloride Channel을 통한 Chloride 분비 활성화

DC Field Value Language
dc.contributor.author이민구-
dc.contributor.author이원상-
dc.contributor.author지헌영-
dc.contributor.author최재영-
dc.contributor.author손은진-
dc.date.accessioned2014-12-20T17:37:44Z-
dc.date.available2014-12-20T17:37:44Z-
dc.date.issued2011-
dc.identifier.issn2092-5859-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/94962-
dc.description.abstractBackground and ObjectivesZZNucleotide binding to purinergic P2Y receptors contributes to the regulation of fluid and ion transport in the middle ear epithelial cells. Here, we investigated the regulatory mechanism of the P2Y2 receptor agonist, uridine-5’-triphosphate (UTP), on Cl- transport in cultured normal human middle ear epithelial (NHMEE) cells. Materials and MethodZZElectrophysiological measurements were performed in monolayers of cultured NHMEE cells. Short circuit currents (Isc) were measured from the cells mounted in Ussing chambers under various conditions. ResultsZZApical addition of UTP in presence of amiloride evoked a transient rise and a sustained response in Isc due to Cl- efflux. Application of different Cl- channel blockers to the apical side of the cells significantly decreased UTP-induced Isc. Niflumic acid (NFA), a known blocker of Ca2+-activated chloride channels (CACC), and CFTRinh172, a selective inhibitor of cystic fibrosis transmembrane conductance regulator (CFTR), partially inhibited the UTP-induced Cl- secretion, respectively. ConclusionZZCl- transport across the airway epithelia plays a predominant role in regulating airway hydration. In this study, UTP is shown to increase both CACC and CFTR-dependent Cl- secretion in NHMEE cells, suggesting their role in fluid and ion transport in the middle ear epithelium-
dc.description.statementOfResponsibilityopen-
dc.format.extent840~846-
dc.relation.isPartOfKorean Journal of Otorhinolaryngology-Head and Neck Surgery-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.title사람중이점막세포에서 Uridine-5’-Triphosphate 자극에 의한 Cystic Fibrosis Transmembrane Conductance Regulator와 Ca2+-Activated Chloride Channel을 통한 Chloride 분비 활성화-
dc.title.alternativeUridine-5’-Triphosphate Stimulates Chloride Secretion via Cystic Fibrosis Transmembrane Conductance Regulator and Ca2+-Activated Chloride Channels in Cultured Human Middle Ear Epithelial Cells-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorEun Jin Son-
dc.contributor.googleauthorHeon Young Gee-
dc.contributor.googleauthorMin-Goo Lee-
dc.contributor.googleauthorWon-Sang Lee-
dc.contributor.googleauthorJae Young Choi-
dc.identifier.doi10.3342/kjorl-hns.2011.54.12.840-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA04173-
dc.contributor.localIdA02781-
dc.contributor.localIdA03000-
dc.contributor.localIdA03971-
dc.contributor.localIdA01989-
dc.relation.journalcodeJ02089-
dc.subject.keywordUTP-
dc.subject.keywordChloride-
dc.subject.keywordIon channel-
dc.subject.keywordMiddle ear-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.alternativeNameLee, Won Sang-
dc.contributor.alternativeNameGee, Heon Yung-
dc.contributor.alternativeNameChoi, Jae Young-
dc.contributor.alternativeNameSon, Eun Jin-
dc.contributor.affiliatedAuthorChoi, Jae Young-
dc.contributor.affiliatedAuthorLee, Min Goo-
dc.contributor.affiliatedAuthorLee, Won Sang-
dc.contributor.affiliatedAuthorGee, Heon Yung-
dc.contributor.affiliatedAuthorSon, Eun Jin-
dc.rights.accessRightsfree-
dc.citation.volume54-
dc.citation.number12-
dc.citation.startPage840-
dc.citation.endPage846-
dc.identifier.bibliographicCitationKorean Journal of Otorhinolaryngology-Head and Neck Surgery, Vol.54(12) : 840-846, 2011-
dc.identifier.rimsid26949-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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