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Decrease of GABAergic markers and arc protein expression in the frontal cortex by intraventricular 192 IgG-saporin.

 Jeong D.U  ;  Chang W.S.  ;  Chang J.W  ;  Lee D.  ;  Hwang Y.S. 
 Dementia and Geriatric Cognitive Disorders, Vol.32(1) : 70-78, 2011 
Journal Title
 Dementia and Geriatric Cognitive Disorders 
Issue Date
BACKGROUND/AIMS: Previous studies used 192 IgG-saporin to study cholinergic function because of its facility for selective lesioning; however, results varied due to differences in the methods of administration and behavioral tests used. We examined an animal model of dementia using 192 IgG-saporin to confirm its features before applying this model to research of therapeutic drugs or electrical stimulation techniques. METHODS: Features were verified by the Morris water maze test, immunochemistry, and Western blotting. Animals were examined after intraventricular injection of 192 IgG-saporin (0.63 μg/μl; 6, 8, and 10 μl) or phosphate-buffered saline. RESULTS: In the acquisition phase of the Morris water maze test, the latencies of the injection groups were significantly delayed, but recovered within 1 week. In the probe test, 2 of 4 indices (time in the platform zone and the number of crossings) were significantly different in the 8-μl injection group. Immunohistochemistry revealed the extent of cholinergic destruction. Activity-regulated cytoskeleton-associated protein and glutamate decarboxylase expression significantly decreased in the frontal cortex (8- and 10-μl groups), but not in the hippocampus. CONCLUSION: Spatial memory impairment was associated with cholinergic basal forebrain injury as well as frontocortical GABAergic hypofunction and synaptic plasticity deceleration.
Appears in Collections:
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
Yonsei Authors
이동규(Lee, Dongkyu) ; 장원석(Chang, Won Seok) ; 장진우(Chang, Jin Woo) ; 정다운(Jeong, Da Un) ; 황용섭(Hwang, Yong Sup)
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