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Ribosomal protein S3 is phosphorylated by Cdk1/cdc2 during G2/M phase

Authors
 In-Soo Yoon ; Ji Hyung Chung ; Ye Sun Han ; Joon Kim ; Tae-Sung Kim ; Lin-Woo Kang ; Sung Il Ko ; You Ri Lee ; Min Ju Park ; Soo-Hyun Hahm 
Citation
 BMB Reports, Vol.44(8) : 529~534, 2011 
Journal Title
 BMB Reports 
ISSN
 1976-6696 
Issue Date
2011
Abstract
Ribosomal protein S3 (rpS3) is a multifunctional protein involved in translation, DNA repair, and apoptosis. The relationship between rpS3 and cyclin-dependent kinases (Cdks) involved in cell cycle regulation is not yet known. Here, we show that rpS3 is phosphorylated by Cdk1 in G2/M phase. Co-immunoprecipitation and GST pull-down assays revealed that Cdk1 interacted with rpS3. An in vitro kinase assay showed that Cdk1 phosphorylated rpS3 protein. Phosphorylation of rpS3 increased in nocodazole-arrested mitotic cells; however, treatment with Cdk1 inhibitor or Cdk1 siRNA significantly attenuated this phosphorylation event. The phosphorylation of a mutant form of rpS3, T221A, was significantly reduced compared with wild-type rpS3. Decreased phosphorylation and nuclear accumulation of T221A was much more pronounced in G2/M phase. These results suggest that the phosphorylation of rpS3 by Cdk1 occurs at Thr221 during G2/M phase and, moreover, that this event is important for nuclear accumulation of rpS3.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94068
DOI
10.5483/BMBRep.2011.44.8.529
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Cardiovascular Product Evaluation Center
Yonsei Authors
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