Cited 21 times in
Ribosomal protein S3 is phosphorylated by Cdk1/cdc2 during G2/M phase
DC Field | Value | Language |
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dc.contributor.author | 정지형 | - |
dc.date.accessioned | 2014-12-20T17:09:32Z | - |
dc.date.available | 2014-12-20T17:09:32Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1976-6696 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/94068 | - |
dc.description.abstract | Ribosomal protein S3 (rpS3) is a multifunctional protein involved in translation, DNA repair, and apoptosis. The relationship between rpS3 and cyclin-dependent kinases (Cdks) involved in cell cycle regulation is not yet known. Here, we show that rpS3 is phosphorylated by Cdk1 in G2/M phase. Co-immunoprecipitation and GST pull-down assays revealed that Cdk1 interacted with rpS3. An in vitro kinase assay showed that Cdk1 phosphorylated rpS3 protein. Phosphorylation of rpS3 increased in nocodazole-arrested mitotic cells; however, treatment with Cdk1 inhibitor or Cdk1 siRNA significantly attenuated this phosphorylation event. The phosphorylation of a mutant form of rpS3, T221A, was significantly reduced compared with wild-type rpS3. Decreased phosphorylation and nuclear accumulation of T221A was much more pronounced in G2/M phase. These results suggest that the phosphorylation of rpS3 by Cdk1 occurs at Thr221 during G2/M phase and, moreover, that this event is important for nuclear accumulation of rpS3. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 529~534 | - |
dc.relation.isPartOf | BMB REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | CDC2 Protein Kinase | - |
dc.subject.MESH | Cell Division* | - |
dc.subject.MESH | Cell Nucleus/metabolism | - |
dc.subject.MESH | Cyclin B/metabolism* | - |
dc.subject.MESH | Cyclin-Dependent Kinases | - |
dc.subject.MESH | G2 Phase* | - |
dc.subject.MESH | HEK293 Cells | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Phosphothreonine/metabolism | - |
dc.subject.MESH | Protein Binding | - |
dc.subject.MESH | Ribosomal Proteins/metabolism* | - |
dc.title | Ribosomal protein S3 is phosphorylated by Cdk1/cdc2 during G2/M phase | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Research Institute for Cerebral & Cardiovascular Diseases (심혈관제품유효성평가센터) | - |
dc.contributor.googleauthor | In-Soo Yoon | - |
dc.contributor.googleauthor | Ji Hyung Chung | - |
dc.contributor.googleauthor | Soo-Hyun Hahm | - |
dc.contributor.googleauthor | Min Ju Park | - |
dc.contributor.googleauthor | You Ri Lee | - |
dc.contributor.googleauthor | Sung Il Ko | - |
dc.contributor.googleauthor | Lin-Woo Kang | - |
dc.contributor.googleauthor | Tae-Sung Kim | - |
dc.contributor.googleauthor | Joon Kim | - |
dc.contributor.googleauthor | Ye Sun Han | - |
dc.identifier.doi | 10.5483/BMBRep.2011.44.8.529 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03739 | - |
dc.relation.journalcode | J00348 | - |
dc.identifier.eissn | 1976-670X | - |
dc.identifier.pmid | 21871177 | - |
dc.subject.keyword | Cdk1/cdc2 | - |
dc.subject.keyword | Cell cycle | - |
dc.subject.keyword | Phosphorylation | - |
dc.subject.keyword | RpS3 | - |
dc.contributor.alternativeName | Chung, Ji Hyung | - |
dc.contributor.affiliatedAuthor | Chung, Ji Hyung | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 44 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 529 | - |
dc.citation.endPage | 534 | - |
dc.identifier.bibliographicCitation | BMB REPORTS, Vol.44(8) : 529-534, 2011 | - |
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