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Vascular tube formation and angiogenesis induced by polyvinylpyrrolidone-coated silver nanoparticles

Authors
 Kyeongah Kang  ;  Dae-Hyoun Lim  ;  In-Hong Choi  ;  Taegyeong Kang  ;  Kangtaek Lee  ;  Eun-Yi Moon  ;  Young Yang  ;  Myeong-Sok Lee  ;  Jong-Seok Lim 
Citation
 TOXICOLOGY LETTERS, Vol.205(3) : 227-234, 2011 
Journal Title
 TOXICOLOGY LETTERS 
ISSN
 0378-4274 
Issue Date
2011
MeSH
Animals ; Cell Line ; Drug Implants ; Endothelium, Vascular/drug effects* ; Endothelium, Vascular/metabolism ; Female ; Hemoglobins/metabolism ; Melanoma, Experimental/blood supply ; Melanoma, Experimental/metabolism ; Metal Nanoparticles/administration & dosage ; Metal Nanoparticles/chemistry ; Metal Nanoparticles/toxicity* ; Mice ; Mice, Inbred C57BL ; Neovascularization, Pathologic/chemically induced* ; Neovascularization, Pathologic/metabolism ; Nitric Oxide/metabolism ; Particle Size ; Povidone/administration & dosage ; Povidone/chemistry ; Povidone/toxicity* ; Reactive Oxygen Species/metabolism ; Receptors, Vascular Endothelial Growth Factor/metabolism ; Signal Transduction/drug effects ; Silver/administration & dosage ; Silver/chemistry ; Silver/toxicity* ; Subcutaneous Tissue/blood supply ; Subcutaneous Tissue/drug effects ; Subcutaneous Tissue/metabolism ; Subcutaneous Tissue/pathology ; Surface Properties ; Vascular Endothelial Growth Factors/metabolism
Keywords
Silver nanoparticles ; Matrigel plug ; Angiogenesis ; Vascular endothelial growth factor ; VEGF signaling
Abstract
Silver nanoparticles (AgNPs) are one of the most commonly used nanomaterials due to their antibacterial properties. In this study, we examined the effects of polyvinylpyrrolidone (PVP)-coated AgNPs (average size 2.3nm) on angiogenesis in both an in vivo model and an in vitro endothelial cell line, SVEC4-10. Increased angiogenesis was detected around the injection site of AgNP-containing Matrigel in vivo. AgNPs also increased the infiltration of endothelial cells and the hemoglobin (Hb) content in AgNP-Matrigel plugs implanted into mice. AgNPs induced endothelial cell tube formation on growth factor-reduced Matrigel, production of reactive oxygen species (ROS), and production of angiogenic factors, such as vascular endothelial growth factor (VEGF) and nitric oxide (NO), in SVEC4-10 cells. In addition, AgNPs promoted the activation of FAK, Akt, ERK1/2, and p38, which are all involved in VEGF receptor (VEGFR)-mediated signaling. Finally, AgNP-treated tumors caused angiogenesis around tumors in B16F10 melanomas after they were injected into mice, and the Hb concentration in the tumors increased in a concentration-dependent manner with AgNP treatment. Thus, our study suggests that exposure to AgNPs can cause angiogenesis through the production of angiogenic factors.
Full Text
http://www.sciencedirect.com/science/article/pii/S0378427411012513
DOI
10.1016/j.toxlet.2011.05.1033
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93990
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