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Single-molecule recognition of biomolecular interaction via Kelvin probe force microscopy

Authors
 Jinsung Park  ;  Jaemoon Yang  ;  Gyudo Lee  ;  Chang Young Lee  ;  Sungsoo Na  ;  Sang Woo Lee  ;  Seungjoo Haam  ;  Yong-Min Huh  ;  Dae Sung Yoon  ;  Kilho Eom  ;  Taeyun Kwon 
Citation
 ACS NANO, Vol.5(9) : 6981-6990, 2011 
Journal Title
 ACS NANO 
ISSN
 1936-0851 
Issue Date
2011
MeSH
Microscopy, Scanning Probe/methods* ; Protein-Tyrosine Kinases/metabolism
Keywords
biomolecular interactions ; Kelvin probe force microscopy ; label-free ; protein kinase ; single molecule ; surface potential
Abstract
We report the scanning probe microscope (SPM)-based single-molecule recognition of biomolecular interactions between protein kinase and small ligands (i.e., ATP and Imatinib). In general, it is difficult to sense and detect the small ligands bound to protein kinase (at single-molecule resolution) using a conventional atomic force microscope (AFM) due to the limited resolution of conventional AFM for detecting the miniscule changes in molecular size driven by ligand binding. In this study, we have demonstrated that Kelvin probe force microscopy (KPFM) is able to articulate the surface potential of biomolecules interacting with ligands (i.e., the protein kinase-ATP interactions and inhibition phenomena induced by antagonistic molecules) in a label-free manner. Furthermore, measured surface potentials for biomolecular interactions enable quantitative descriptions on the ability of protein kinase to interact with small ligands such as ATP or antagonistic molecules. Our study sheds light on KPFM that allows the precise recognition of single-molecule interactions, which opens a new avenue for the design and development of novel molecular therapeutics.
Full Text
http://pubs.acs.org/doi/abs/10.1021/nn201540c
DOI
10.1021/nn201540c
Appears in Collections:
5. Research Institutes (연구소) > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93616
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