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Melatonin protects against oxidative stress in granular corneal dystrophy type 2 corneal fibroblasts by mechanisms that involve membrane melatonin receptors

Authors
 Seung-Il Choi  ;  Shorafidinkhuja Dadakhujaev  ;  Hyunmi Ryu  ;  Tae im Kim  ;  Eung Kweon Kim 
Citation
 Journal of Pineal Research, Vol.51(1) : 94-103, 2011 
Journal Title
 Journal of Pineal Research 
ISSN
 0742-3098 
Issue Date
2011
Abstract
Considering that oxidative stress plays a role in corneal fibroblast degeneration during granular corneal dystrophy type 2 (GCD2) and melatonin is an effective antioxidant, we examined the ability of melatonin to protect against oxidative stress-induced cell death of primary cultured normal and GCD2-homozygous corneal fibroblasts. Melatonin treatment protected primary cultured normal and GCD2 corneal fibroblasts from paraquat (PQ)-induced oxidative stress and caused increased expression levels of Cu/Zn-superoxide dismutase (SOD1) and glutathione reductase (GR) in both types of cells. Interestingly, catalase expression increased in normal corneal fibroblasts, but decreased in GCD2 corneal fibroblasts after melatonin treatment. Melatonin also reduced the levels of intracellular reactive oxygen species and H(2)O(2) in both cell types. In addition, the selective melatonin receptor antagonist luzindole blocked melatonin-induced expression of SOD1 and GR. The expression levels of melatonin receptors 1A (MT1) and 1B (MT2) were significantly higher in GCD2 corneal fibroblasts than in normal cells. These results suggest that increased expression of melatonin receptors may be involved in the defense mechanisms against oxidative stress in GCD2 corneal fibroblasts, and melatonin may have potential therapeutic implications for GCD2 treatment.
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93493
DOI
10.1111/j.1600-079X.2011.00866.x
Appears in Collections:
1. Journal Papers (연구논문) > 5. Research Institutes (연구소) > Corneal Dystrophy Research Institute (각막이상증연구소)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원)
1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Ophthalmology (안과학교실)
Yonsei Authors
김응권(Kim, Eung Kweon) ; 김태임(Kim, Tae Im) ; 다다후자예프(Dadakhujaev, Shorafidinkhuja) ; 류현미(Ryu, Hyun Mi) ; 최승일(Choi, Seung Il)
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Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-079X.2011.00866.x/abstract
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