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Effect of seeding using an avidin-biotin binding system on the attachment of periodontal ligament fibroblasts to nanohydroxyapatite scaffolds: three-dimensional culture.

Authors
 Yong-Ju Jang  ;  Im-Hee Jung  ;  Jung-Chul Park  ;  Ui-Won Jung  ;  Chang-Sung Kim  ;  Yong-Keun Lee  ;  Chong-Kwan Kim  ;  Seong-Ho Choi 
Citation
 JOURNAL OF PERIODONTAL AND IMPLANT SCIENCE, Vol.41(2) : 73-78, 2011 
Journal Title
JOURNAL OF PERIODONTAL AND IMPLANT SCIENCE
ISSN
 2093-2278 
Issue Date
2011
Keywords
Cell adhesion ; Fibroblasts ; Periodontal ligament ; Tissue engineering
Abstract
PURPOSE: For periodontal tissue engineering, it is a primary requisite and a challenge to select the optimum types of cells, properties of scaffold, and growth factor combination to reconstruct a specific tissue in its natural form and with the appropriate function. Owing to fundamental disadvantages associated with using a two-dimensional substrate, several methods of seeding cells into three-dimensional scaffolds have been reported and the authors have asserted its usefulness and effectiveness. In this study, we explore the cell attachment of periodontal ligament fibroblasts on nanohydroxyapatite (n-HA) scaffold using avidin biotin binding system (ABBS).

METHODS: Human periodontal ligament fibroblasts were isolated from the health tooth extracted for the purpose of orthodontic procedure. HA nanoparticles were prepared and Ca(NO(3))(2)-4H(2)O and (OC(2)H(5))(3)P were selected as precursors of HA sol. The final scaffold was 8 mm in diameter and 3 mm in height disk with porosity value of 81.55%. 1×10(5) periodontal ligament fibroblasts were applied to each scaffold. The cells were seeded into scaffolds by static, agitating and ABBS seeding method.

RESULTS: The number of periodontal ligament fibroblasts attached was greater for ABBS seeding method than for static or agitating method (P<0.05). No meaningful difference has been observed among seeding methods with scanning electron microscopy images. However, increased strength of cell attachment of ABBS could be deduced from the high affinity between avidin and biotin (Kd=10(-15) M).

CONCLUSIONS: The high-affinity ABBS enhances the ability of periodontal ligament fibroblasts to attach to three-dimensionally constructed n-HA scaffolds.
Files in This Item:
T201101516.pdf Download
DOI
10.5051/jpis.2011.41.2.73
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Dental Biomaterials and Bioengineering (치과생체재료공학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chong Kwan(김종관)
Kim, Chang Sung(김창성) ORCID logo https://orcid.org/0000-0003-3902-1071
Park, Jung Chul(박정철)
Lee, Yong Keun(이용근)
Jung, Ui Won(정의원) ORCID logo https://orcid.org/0000-0001-6371-4172
Jung, Im Hee(정임희)
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93314
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