Cited 13 times in
α-Melanocyte-stimulating hormone inhibits tumor necrosis factor α-stimulated MUC5AC expression in human nasal epithelial cells.
DC Field | Value | Language |
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dc.contributor.author | 주정희 | - |
dc.contributor.author | 최연호 | - |
dc.contributor.author | 김규보 | - |
dc.contributor.author | 김윤주 | - |
dc.contributor.author | 유지환 | - |
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 이상남 | - |
dc.contributor.author | 이현재 | - |
dc.date.accessioned | 2014-12-20T16:44:57Z | - |
dc.date.available | 2014-12-20T16:44:57Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1044-1549 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93304 | - |
dc.description.abstract | Mucin hypersecretion is an important clinical feature of several respiratory diseases, including asthma, cystic fibrosis, nasal allergy, rhinitis, and sinusitis. It has been shown that α-melanocyte-stimulating hormone (α-MSH), a proopiomelanocortin (POMC)-derived peptide, has immunomodulatory activities by inhibiting NF-κB activation induced by proinflammatory cytokines such as TNF-α. Because MUC5AC expression is known to be up-regulated by TNF-α via NF-κB activation, we evaluated the inhibitory effect of α-MSH on MUC5AC gene expression induced by TNF-α in normal human nasal epithelial (NHNE) cells. Melanocortin-1-receptor (MC-1R) was detected by RT-PCR, Western blotting, and immunofluorescent labeling in NHNE cells. α-MSH suppressed NF-κB/p65 phosphorylation induced by TNF-α as well as IkB-α degradation in a dose-dependent manner, as assessed by Western blotting. In addition, α-MSH inhibited TNF-α-induced nuclear translocation of NF-κB and NF-κB luciferase activity. Real-time quantitative PCR data showed that α-MSH inhibited TNF-α-induced expression of MUC5AC, and this effect of α-MSH was neutralized by knockdown of MC-1R using MC-1R shRNA lentivirus. Analyses using RT-PCR and Western blotting showed the expression of POMC and two key enzymes in the POMC processing, proprotein convertases (PC)1 and PC2, and 7B2, which is required for enzymatic activity of PC2, in normal human nasal mucosa. We conclude that α-MSH down-regulates MUC5AC expression by inhibiting TNF-α-induced NF-κB activity through MC-1R stimulation in NHNE cells and that normal human nasal mucosa possesses the POMC processing machinery. Therefore, α-MSH may be a promising candidate to decrease mucin overproduction initiated by NF-κB activation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 716~724 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Active Transport, Cell Nucleus | - |
dc.subject.MESH | Epithelial Cells/cytology | - |
dc.subject.MESH | Epithelial Cells/metabolism* | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | I-kappa B Proteins/metabolism | - |
dc.subject.MESH | Lentivirus/genetics | - |
dc.subject.MESH | Lentivirus/metabolism | - |
dc.subject.MESH | Melanocytes/metabolism* | - |
dc.subject.MESH | Models, Biological | - |
dc.subject.MESH | Mucin 5AC/antagonists & inhibitors | - |
dc.subject.MESH | Mucin 5AC/metabolism* | - |
dc.subject.MESH | NF-KappaB Inhibitor alpha | - |
dc.subject.MESH | NF-kappa B/metabolism | - |
dc.subject.MESH | Nose/metabolism* | - |
dc.subject.MESH | Pro-Opiomelanocortin/metabolism | - |
dc.subject.MESH | Receptor, Melanocortin, Type 1/metabolism | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/antagonists & inhibitors | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/metabolism* | - |
dc.subject.MESH | alpha-MSH/metabolism | - |
dc.title | α-Melanocyte-stimulating hormone inhibits tumor necrosis factor α-stimulated MUC5AC expression in human nasal epithelial cells. | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | 생체방어연구센터 | - |
dc.contributor.googleauthor | Sang-Nam Lee | - |
dc.contributor.googleauthor | Ji-Hwan Ryu | - |
dc.contributor.googleauthor | Jung-Hee Joo | - |
dc.contributor.googleauthor | Yeon-Ho Choi | - |
dc.contributor.googleauthor | Hyun-Jae Lee | - |
dc.contributor.googleauthor | Yoon-Ju Kim | - |
dc.contributor.googleauthor | Kyu-Bo Kim | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.identifier.doi | 10.1165/rcmb.2009-0420OC | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03958 | - |
dc.contributor.localId | A04110 | - |
dc.contributor.localId | A00323 | - |
dc.contributor.localId | A00795 | - |
dc.contributor.localId | A02604 | - |
dc.contributor.localId | A02813 | - |
dc.contributor.localId | A03293 | - |
dc.contributor.localId | A02522 | - |
dc.relation.journalcode | J00113 | - |
dc.identifier.eissn | 1535-4989 | - |
dc.identifier.pmid | 20639461 | - |
dc.identifier.url | http://www.atsjournals.org/doi/abs/10.1165/rcmb.2009-0420OC?journalCode=ajrcmb | - |
dc.subject.keyword | [alpha]-MSH | - |
dc.subject.keyword | mucin | - |
dc.subject.keyword | MC-1R | - |
dc.subject.keyword | NF-[kappa]B pathway | - |
dc.subject.keyword | TNF-[alpha] | - |
dc.contributor.alternativeName | Joo, Jung Hee | - |
dc.contributor.alternativeName | Choi, Yeon Ho | - |
dc.contributor.alternativeName | Kim, Kyu Bo | - |
dc.contributor.alternativeName | Kim, Yoon Ju | - |
dc.contributor.alternativeName | Ryu, Ji Hwan | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.alternativeName | Lee, Sang Nam | - |
dc.contributor.alternativeName | Lee, Hyun Jae | - |
dc.contributor.affiliatedAuthor | Joo, Jung Hee | - |
dc.contributor.affiliatedAuthor | Choi, Yeon Ho | - |
dc.contributor.affiliatedAuthor | Kim, Kyu Bo | - |
dc.contributor.affiliatedAuthor | Kim, Yoon Ju | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Lee, Sang Nam | - |
dc.contributor.affiliatedAuthor | Lee, Hyun Jae | - |
dc.contributor.affiliatedAuthor | Ryu, Ji Hwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 44 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 716 | - |
dc.citation.endPage | 724 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, Vol.44(5) : 716-724, 2011 | - |
dc.identifier.rimsid | 27121 | - |
dc.type.rims | ART | - |
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