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Crosstalk between platelet-derived growth factor-induced Nox4 activation and MUC8 gene overexpression in human airway epithelial cells

Authors
 Hyun Jik Kim  ;  Chang-Hoon Kim  ;  Ji-Hwan Ryu  ;  Jung Hee Joo  ;  Sang-Nam Lee  ;  Min-Ji Kim  ;  Jeung-Gweon Lee  ;  Yun Soo Bae  ;  Joo-Heon Yoon 
Citation
 FREE RADICAL BIOLOGY AND MEDICINE, Vol.50(9) : 1039-1052, 2011 
Journal Title
FREE RADICAL BIOLOGY AND MEDICINE
ISSN
 0891-5849 
Issue Date
2011
MeSH
Adenoviridae ; Blotting, Western ; Cell Culture Techniques ; Chronic Disease ; Enzyme Activation ; Epithelial Cells/enzymology ; Epithelial Cells/pathology ; Gene Expression Regulation ; Humans ; Isoenzymes/genetics ; Isoenzymes/metabolism ; Mucins/genetics ; Mucins/metabolism* ; NADPH Oxidase 4 ; NADPH Oxidases/genetics ; NADPH Oxidases/metabolism* ; Nasal Mucosa/metabolism ; Nasal Mucosa/physiopathology ; Oxidative Stress ; Platelet-Derived Growth Factor/genetics ; Platelet-Derived Growth Factor/metabolism* ; RNA Interference ; RNA, Small Interfering/metabolism ; Reactive Oxygen Species/analysis ; Reactive Oxygen Species/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction* ; Sinusitis/genetics ; Sinusitis/metabolism* ; Sinusitis/physiopathology ; Transduction, Genetic ; Tumor Cells, Cultured
Keywords
Platelet-derived growth factor ; MUC8 ; Nox4 ; Reactive oxygen species ; Free radicals
Abstract
Reactive oxygen species (ROS) contribute to chronic airway inflammation, and NADPH oxidase (Nox) is an important source of ROS. However, little is known of the role that ROS play in chronic upper respiratory tract inflammation. We investigated the mechanism of ROS generation and its association with mucin gene overexpression in the nasal epithelium. The level of platelet-derived growth factor (PDGF) expression was increased in sinusitis mucosa, and high-level PDGF expression induced intracellular ROS, followed by MUC8 gene overexpression in normal human nasal epithelial cells. Knockdown of Nox4 expression with Nox4 siRNA decreased PDGF-induced intracellular ROS and MUC8 expression. Infection with an adenovirus containing Nox4 cDNA resulted in Nox4 overexpression and increased intracellular levels of ROS and MUC8 expression. PDGF and Nox4 overexpression are essential components of intracellular ROS generation and may contribute to chronic inflammation in the nasal epithelium through induction of MUC8 overexpression.
Full Text
http://www.sciencedirect.com/science/article/pii/S0891584911000347
DOI
10.1016/j.freeradbiomed.2011.01.014
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chang Hoon(김창훈) ORCID logo https://orcid.org/0000-0003-1238-6396
Ryu, Ji Hwan(유지환)
Yoon, Joo Heon(윤주헌)
Lee, Sang Nam(이상남)
Lee, Jeung Gweon(이정권)
Joo, Jung Hee(주정희)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93299
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