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Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells

 Hong Seo Choi  ;  Won-Tae Kim  ;  Hana Kim  ;  Jum-Ji Kim  ;  Ji-Yun Ko  ;  Sang-Wang Lee  ;  Young Joo Jang  ;  Sang Jick Kim  ;  Min-Jung Lee  ;  Han-Sung Jung  ;  Julia Kzhyshkowska  ;  Soo-Jong Um  ;  Mi-Young Lee  ;  Sang-Hun Lee  ;  Cheorl-Ho Kim  ;  Chun Jeih Ryu 
 STEM CELLS AND DEVELOPMENT, Vol.20(4) : 609-620, 2011 
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Issue Date
Amino Acid Sequence ; Animals ; Antigens, Differentiation/metabolism* ; Cell Differentiation ; Cell Line ; Cell Line, Tumor ; Coculture Techniques ; Down-Regulation ; Embryonic Stem Cells/cytology ; Embryonic Stem Cells/metabolism* ; Flow Cytometry ; Heterogeneous-Nuclear Ribonucleoproteins/genetics ; Heterogeneous-Nuclear Ribonucleoproteins/metabolism* ; Homeodomain Proteins/metabolism ; Humans ; Immunoprecipitation ; Membrane Proteins/metabolism* ; Mice ; Molecular Sequence Data ; Nanog Homeobox Protein ; Neoplastic Stem Cells/metabolism ; Nuclear Proteins/genetics ; Nuclear Proteins/metabolism* ; Phosphorylation ; Pluripotent Stem Cells/cytology ; Pluripotent Stem Cells/metabolism ; SOXB1 Transcription Factors/metabolism ; Transcription Factors/genetics ; Transcription Factors/metabolism*
Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs.
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2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Lee, Min Jung(이민정)
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
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