Cited 21 times in
Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells
DC Field | Value | Language |
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dc.contributor.author | 이민정 | - |
dc.contributor.author | 정한성 | - |
dc.date.accessioned | 2014-12-20T16:29:10Z | - |
dc.date.available | 2014-12-20T16:29:10Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1547-3287 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/92805 | - |
dc.description.abstract | Pluripotent human embryonic stem cells (hESCs) provide appropriate systems for developmental studies and prospective donor cell sources for regenerative medicine. Identification of surface markers specific to hESCs is a prerequisite for studying hESC biology and can be used to generate clinical-level donor cell preparations that are free from tumorigenic undifferentiated hESCs. We previously reported the generation of monoclonal antibodies that specifically recognize hESC surface antigens using a decoy immunization strategy. In this study, we show that monoclonal antibody 57-C11 recognizes a phosphorylated form of adenovirus early region 1B-associated protein 5 (E1B-AP5). E1B-AP5 is a nuclear RNA-binding protein, but we report that 57-C11-reactive E1B-AP5 is expressed on the surface of undifferentiated hESCs. In undifferentiated hESCs, 57-C11-reactive E1B-AP5 is localized to SSEA3-, SSEA4-, TRA-1-60-, TRA-1-81-, OCT4-, SOX2-, and NANOG-positive hESCs. In mixtures of undifferentiated hESCs and hESC-derived neurons, 57-C11 exclusively recognizes undifferentiated hESCs but not hESC-derived neuronal cells. Further, the expression of 57-C11-reactive E1B-AP5 decreases upon differentiation. Our results demonstrate that 57-C11-reactive E1B-AP5 is a novel surface molecule that is involved in the undifferentiated state of hESCs. As far as we know, this is the first report demonstrating that heterogeneous nuclear RNA-binding protein is expressed on the surface of undifferentiated hESCs. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 609~620 | - |
dc.relation.isPartOf | STEM CELLS AND DEVELOPMENT | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antigens, Differentiation/metabolism* | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Coculture Techniques | - |
dc.subject.MESH | Down-Regulation | - |
dc.subject.MESH | Embryonic Stem Cells/cytology | - |
dc.subject.MESH | Embryonic Stem Cells/metabolism* | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | Heterogeneous-Nuclear Ribonucleoproteins/genetics | - |
dc.subject.MESH | Heterogeneous-Nuclear Ribonucleoproteins/metabolism* | - |
dc.subject.MESH | Homeodomain Proteins/metabolism | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoprecipitation | - |
dc.subject.MESH | Membrane Proteins/metabolism* | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Nanog Homeobox Protein | - |
dc.subject.MESH | Neoplastic Stem Cells/metabolism | - |
dc.subject.MESH | Nuclear Proteins/genetics | - |
dc.subject.MESH | Nuclear Proteins/metabolism* | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Pluripotent Stem Cells/cytology | - |
dc.subject.MESH | Pluripotent Stem Cells/metabolism | - |
dc.subject.MESH | SOXB1 Transcription Factors/metabolism | - |
dc.subject.MESH | Transcription Factors/genetics | - |
dc.subject.MESH | Transcription Factors/metabolism* | - |
dc.title | Identification and characterization of adenovirus early region 1B-associated protein 5 as a surface marker on undifferentiated human embryonic stem cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Hong Seo Choi | - |
dc.contributor.googleauthor | Won-Tae Kim | - |
dc.contributor.googleauthor | Hana Kim | - |
dc.contributor.googleauthor | Jum-Ji Kim | - |
dc.contributor.googleauthor | Ji-Yun Ko | - |
dc.contributor.googleauthor | Sang-Wang Lee | - |
dc.contributor.googleauthor | Young Joo Jang | - |
dc.contributor.googleauthor | Sang Jick Kim | - |
dc.contributor.googleauthor | Min-Jung Lee | - |
dc.contributor.googleauthor | Han-Sung Jung | - |
dc.contributor.googleauthor | Julia Kzhyshkowska | - |
dc.contributor.googleauthor | Soo-Jong Um | - |
dc.contributor.googleauthor | Mi-Young Lee | - |
dc.contributor.googleauthor | Sang-Hun Lee | - |
dc.contributor.googleauthor | Cheorl-Ho Kim | - |
dc.contributor.googleauthor | Chun Jeih Ryu | - |
dc.identifier.doi | 10.1089/scd.2010.0265 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03758 | - |
dc.contributor.localId | A02785 | - |
dc.relation.journalcode | J02684 | - |
dc.identifier.eissn | 1557-8534 | - |
dc.identifier.pmid | 21083500 | - |
dc.identifier.url | http://dx.doi.org/10.1089/scd.2010.0265 | - |
dc.contributor.alternativeName | Lee, Min Jung | - |
dc.contributor.alternativeName | Jung, Han Sung | - |
dc.contributor.affiliatedAuthor | Jung, Han Sung | - |
dc.contributor.affiliatedAuthor | Lee, Min Jung | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 20 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 609 | - |
dc.citation.endPage | 620 | - |
dc.identifier.bibliographicCitation | STEM CELLS AND DEVELOPMENT, Vol.20(4) : 609-620, 2011 | - |
dc.identifier.rimsid | 28768 | - |
dc.type.rims | ART | - |
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