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Quantitative T2* imaging of metastatic human breast cancer to brain in the nude rat at 3 T.

 Ho-Taek Song  ;  Elaine K. Jordan  ;  Bobbi K. Lewis  ;  Eric Gold  ;  Wei Liu  ;  Joseph A. Frank 
 NMR IN BIOMEDICINE, Vol.24(3) : 325-334, 2011 
Journal Title
Issue Date
Animals ; Brain Neoplasms/pathology ; Brain Neoplasms/secondary* ; Breast Neoplasms/pathology* ; Cell Line, Tumor ; Dextrans/metabolism ; Female ; Humans ; Magnetic Resonance Imaging/methods* ; Magnetite Nanoparticles ; Neoplasm Metastasis ; Neoplasm Transplantation ; Protamines/metabolism ; Rats ; Rats, Nude
MRI ; quantification ; cellular tracking ; T2* map ; histogram ; brain metastasis ; breast cancer ; superparamagnetic iron oxides (SPIO)
This study uses quantitative T(2)* imaging to track ferumoxides--protamine sulfate (FEPro)-labeled MDA-MB-231BR-Luc (231BRL) human breast cancer cells that metastasize to the nude rat brain. Four cohorts of nude rats were injected intracardially with FEPro-labeled, unlabeled or tumor necrosis factor-related apoptosis-inducing ligand(TRAIL)-treated (to induce apoptosis) 231BRL cells, or saline, in order to develop metastatic breast cancer in the brain. The heads of the rats were imaged serially over 3-4 weeks using gradient multi-echo and turbo spin-echo pulse sequences at 3 T with a solenoid receive-only 4-cm-diameter coil. Quantitative T(2)* maps of the whole brain were obtained by the application of single-exponential fitting to the signal intensity of T(2)* images, and the distribution of T(2)* values in brain voxels was calculated. MRI findings were correlated with Prussian blue staining and immunohistochemical staining for iron in breast cancer and macrophages. Quantitative analysis of T(2)* from brain voxels demonstrated a significant shift to lower values following the intracardiac injection of FEPro-labeled 231BRL cells, relative to animals receiving unlabeled cells, apoptotic cells or saline. Quartile analysis based on the T(2)* distribution obtained from brain voxels demonstrated significant differences (p < 0.0083) in the number of voxels with T(2)* values in the ranges 10-35  ms (Q1), 36-60  ms (Q2) and 61-86  ms (Q3) from 1 day to 3 weeks post-infusion of labeled 231BRL cells, compared with baseline scans. There were no significant differences in the distribution of T(2)* obtained from serial MRI in rats receiving unlabeled or TRAIL-treated cells or saline. Histologic analysis demonstrated isolated Prussian blue-positive breast cancer cells scattered in the brains of rats receiving labeled cells, relative to animals receiving unlabeled or apoptotic cells. Quantitative T(2)* analysis of FEPro-labeled metastasized cancer cells was possible even after the hypointense voxels were no longer visible on T(2)*-weighted images.
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1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Song, Ho Taek(송호택) ORCID logo https://orcid.org/0000-0002-6655-2575
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