298 682

Cited 0 times in

Developmental Gene Expression Profiling along the Tonotopic Axis of the Mouse Cochlea

Authors
 Eun Jin Son  ;  Ling Wu  ;  Heejei Yoon  ;  Sunhee Kim  ;  Jae Young Choi  ;  Jinwoong Bok 
Citation
 PLOS ONE, Vol.7(7) : e40735, 2012 
Journal Title
PLOS ONE
Issue Date
2012
MeSH
Animals ; Animals, Newborn ; Auditory Perception/genetics* ; Cell Differentiation ; Cluster Analysis ; Cochlea/cytology ; Cochlea/embryology ; Cochlea/growth & development* ; Cochlea/metabolism* ; Embryonic Development/genetics ; Follistatin/genetics ; Follistatin/metabolism ; Gene Expression Profiling* ; Gene Expression Regulation, Developmental* ; Hair Cells, Auditory/cytology ; Hair Cells, Auditory/metabolism ; In Situ Hybridization ; Mice ; Molecular Sequence Annotation ; Nephroblastoma Overexpressed Protein/genetics ; Nephroblastoma Overexpressed Protein/metabolism ; Oligonucleotide Array Sequence Analysis ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; Time Factors
Keywords
Animals ; Animals, Newborn ; Auditory Perception/genetics* ; Cell Differentiation ; Cluster Analysis ; Cochlea/cytology ; Cochlea/embryology ; Cochlea/growth & development* ; Cochlea/metabolism* ; Embryonic Development/genetics ; Follistatin/genetics ; Follistatin/metabolism ; Gene Expression Profiling* ; Gene Expression Regulation, Developmental* ; Hair Cells, Auditory/cytology ; Hair Cells, Auditory/metabolism ; In Situ Hybridization ; Mice ; Molecular Sequence Annotation ; Nephroblastoma Overexpressed Protein/genetics ; Nephroblastoma Overexpressed Protein/metabolism ; Oligonucleotide Array Sequence Analysis ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; Time Factors
Abstract
The mammalian cochlear duct is tonotopically organized such that the basal cochlea is tuned to high frequency sounds and the apical cochlea to low frequency sounds. In an effort to understand how this tonotopic organization is established, we searched for genes that are differentially expressed along the tonotopic axis during neonatal development. Cochlear tissues dissected from P0 and P8 mice were divided into three equal pieces, representing the base, middle and apex, and gene expression profiles were determined using the microarray technique. The gene expression profiles were grouped according to changes in expression levels along the tonotopic axis as well as changes during neonatal development. The classified groups were further analyzed by functional annotation clustering analysis to determine whether genes associated with specific biological function or processes are particularly enriched in each group. These analyses identified several candidate genes that may be involved in cochlear development and acquisition of tonotopy. We examined the expression domains for a few candidate genes in the developing mouse cochlea. Tnc (tenacin C) and Nov (nephroblastoma overexpressed gene) are expressed in the basilar membrane, with increased expression toward the apex, which may contribute to graded changes in the structure of the basilar membrane along the tonotopic axis. In addition, Fst (Follistatin), an antagonist of TGF-β/BMP signaling, is expressed in the lesser epithelial ridge and at gradually higher levels towards the apex. The graded expression pattern of Fst is established at the time of cochlear specification and maintained throughout embryonic and postnatal development, suggesting its possible role in the organization of tonotopy. Our data will provide a good resource for investigating the developmental mechanisms of the mammalian cochlea including the acquisition of tonotopy.
Files in This Item:
T201202728.pdf Download
DOI
22808246
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Anatomy (해부학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Bok, Jin Woong(복진웅) ORCID logo https://orcid.org/0000-0003-1958-1872
Son, Eun Jin(손은진)
Wu, Ling(오령)
Choi, Jae Young(최재영) ORCID logo https://orcid.org/0000-0001-9493-3458
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91703
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links