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Differentially regulated functional gene clusters identified in early hypoxic cardiomyocytes.

 Do Kyun Kim  ;  Eunmi Choi  ;  Byeong-Wook Song  ;  Min-Ji Cha  ;  Onju Ham  ;  Se-Yeon Lee  ;  Chang Youn Lee  ;  Jun-Hee Park  ;  Heesang Song  ;  Ki-Chul Hwang 
 MOLECULAR BIOLOGY REPORTS, Vol.39(10) : 9549-9556, 2012 
Journal Title
Issue Date
Animals ; Calcium-Binding Proteins/genetics ; Calcium-Binding Proteins/metabolism ; Cell Hypoxia ; Cell Proliferation ; Cell Survival ; Cells, Cultured ; Cluster Analysis ; Gene Expression Regulation* ; Ion Channels/genetics ; Ion Channels/metabolism ; Multigene Family* ; Muscle Proteins/genetics ; Muscle Proteins/metabolism ; Myocytes, Cardiac/metabolism* ; Rats ; Rats, Sprague-Dawley ; Transcriptome*
Cardiomyocytes ; Microarray ; Early ischemia ; PKC ; Calcium
Pathological stress including myocardial infarction and hypertension causes a negative effect on calcium regulation and homeostasis. Nevertheless, few studies reveal that Ca(2+) regulatory genes are related to pathological status in cardiomyocytes under early hypoxia. To determine the alteration of Ca(2+)-related gene in hypoxic myocytes, primary neonatal rat ventricular cardiomyocytes (NRVCMs) was isolated. Survival of hypoxic NRVCMs was significantly decreased in 6 h. We confirmed an increase of reactive oxygen species (ROS) generation and Ca(2+) overload in hypoxic NRVCMs by using 2',7'-dichlorodihydro-fluorescein diacetate (H2DCFDA) and FACS analysis. Furthermore, survival/apoptotic signals were also regulated in same condition. The expression profiles of more than 30,000 genes from NRVCMs that were subjected to early hypoxia revealed 630 genes that were differentially regulated. The intracellular Na(+) overload and Ca(2+) handling genes with at least two-fold changes were confirmed. The levels of Ca(2+)-handling proteins (calsequestrin, calmodulin, and calreticulin), ion channels (NCX, Na(+)-K(+)-ATPase, SERCA2a, and PLB), and stress markers (RyR2, ANP, and BNP) were significantly altered in early hypoxia. These results demonstrate that early hypoxia alters Ca(2+)-related gene expression in NRVCMs, leading to pathological status.
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5. Research Institutes (연구소) > Yonsei Cardiovascular Research Institute (심혈관연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
Yonsei Authors
Park, Jun-Hee(박준희)
Song, Byeong Wook(송병욱)
Lee, Se Yeon(이세연)
Lee, Chang Yeon(이창연)
Cha, Min Ji(차민지)
Choi, Eun Mi(최은미)
Ham, On Ju(함온주)
Hwang, Ki Chul(황기철)
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