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Differentially regulated functional gene clusters identified in early hypoxic cardiomyocytes.
DC Field | Value | Language |
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dc.contributor.author | 함온주 | - |
dc.contributor.author | 황기철 | - |
dc.contributor.author | 박준희 | - |
dc.contributor.author | 송병욱 | - |
dc.contributor.author | 이세연 | - |
dc.contributor.author | 이창연 | - |
dc.contributor.author | 차민지 | - |
dc.contributor.author | 최은미 | - |
dc.date.accessioned | 2014-12-19T17:28:09Z | - |
dc.date.available | 2014-12-19T17:28:09Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0301-4851 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91465 | - |
dc.description.abstract | Pathological stress including myocardial infarction and hypertension causes a negative effect on calcium regulation and homeostasis. Nevertheless, few studies reveal that Ca(2+) regulatory genes are related to pathological status in cardiomyocytes under early hypoxia. To determine the alteration of Ca(2+)-related gene in hypoxic myocytes, primary neonatal rat ventricular cardiomyocytes (NRVCMs) was isolated. Survival of hypoxic NRVCMs was significantly decreased in 6 h. We confirmed an increase of reactive oxygen species (ROS) generation and Ca(2+) overload in hypoxic NRVCMs by using 2',7'-dichlorodihydro-fluorescein diacetate (H2DCFDA) and FACS analysis. Furthermore, survival/apoptotic signals were also regulated in same condition. The expression profiles of more than 30,000 genes from NRVCMs that were subjected to early hypoxia revealed 630 genes that were differentially regulated. The intracellular Na(+) overload and Ca(2+) handling genes with at least two-fold changes were confirmed. The levels of Ca(2+)-handling proteins (calsequestrin, calmodulin, and calreticulin), ion channels (NCX, Na(+)-K(+)-ATPase, SERCA2a, and PLB), and stress markers (RyR2, ANP, and BNP) were significantly altered in early hypoxia. These results demonstrate that early hypoxia alters Ca(2+)-related gene expression in NRVCMs, leading to pathological status. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | MOLECULAR BIOLOGY REPORTS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Calcium-Binding Proteins/genetics | - |
dc.subject.MESH | Calcium-Binding Proteins/metabolism | - |
dc.subject.MESH | Cell Hypoxia | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Cell Survival | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cluster Analysis | - |
dc.subject.MESH | Gene Expression Regulation* | - |
dc.subject.MESH | Ion Channels/genetics | - |
dc.subject.MESH | Ion Channels/metabolism | - |
dc.subject.MESH | Multigene Family* | - |
dc.subject.MESH | Muscle Proteins/genetics | - |
dc.subject.MESH | Muscle Proteins/metabolism | - |
dc.subject.MESH | Myocytes, Cardiac/metabolism* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Transcriptome* | - |
dc.title | Differentially regulated functional gene clusters identified in early hypoxic cardiomyocytes. | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Yonsei Cardiovascular Research Institute (심혈관연구소) | - |
dc.contributor.googleauthor | Do Kyun Kim | - |
dc.contributor.googleauthor | Eunmi Choi | - |
dc.contributor.googleauthor | Byeong-Wook Song | - |
dc.contributor.googleauthor | Min-Ji Cha | - |
dc.contributor.googleauthor | Onju Ham | - |
dc.contributor.googleauthor | Se-Yeon Lee | - |
dc.contributor.googleauthor | Chang Youn Lee | - |
dc.contributor.googleauthor | Jun-Hee Park | - |
dc.contributor.googleauthor | Heesang Song | - |
dc.contributor.googleauthor | Ki-Chul Hwang | - |
dc.identifier.doi | 22733491 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A04336 | - |
dc.contributor.localId | A04456 | - |
dc.contributor.localId | A01679 | - |
dc.contributor.localId | A02026 | - |
dc.contributor.localId | A02880 | - |
dc.contributor.localId | A03244 | - |
dc.contributor.localId | A03995 | - |
dc.contributor.localId | A04151 | - |
dc.relation.journalcode | J02249 | - |
dc.identifier.eissn | 1573-4978 | - |
dc.identifier.pmid | 22733491 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs11033-012-1819-1 | - |
dc.subject.keyword | Cardiomyocytes | - |
dc.subject.keyword | Microarray | - |
dc.subject.keyword | Early ischemia | - |
dc.subject.keyword | PKC | - |
dc.subject.keyword | Calcium | - |
dc.contributor.alternativeName | Ham, On Ju | - |
dc.contributor.alternativeName | Hwang, Ki Chul | - |
dc.contributor.alternativeName | Park, Jun-Hee | - |
dc.contributor.alternativeName | Song, Byeong Wook | - |
dc.contributor.alternativeName | Lee, Se Yeon | - |
dc.contributor.alternativeName | Lee, Chang Yeon | - |
dc.contributor.alternativeName | Cha, Min Ji | - |
dc.contributor.alternativeName | Choi, Eun Mi | - |
dc.contributor.affiliatedAuthor | Ham, On Ju | - |
dc.contributor.affiliatedAuthor | Hwang, Ki Chul | - |
dc.contributor.affiliatedAuthor | Park, Jun-Hee | - |
dc.contributor.affiliatedAuthor | Song, Byeong Wook | - |
dc.contributor.affiliatedAuthor | Lee, Se Yeon | - |
dc.contributor.affiliatedAuthor | Lee, Chang Yeon | - |
dc.contributor.affiliatedAuthor | Cha, Min Ji | - |
dc.contributor.affiliatedAuthor | Choi, Eun Mi | - |
dc.citation.volume | 39 | - |
dc.citation.number | 10 | - |
dc.citation.startPage | 9549 | - |
dc.citation.endPage | 9556 | - |
dc.identifier.bibliographicCitation | MOLECULAR BIOLOGY REPORTS, Vol.39(10) : 9549-9556, 2012 | - |
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