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Identification of HnRNP-A2/B1 as a target antigen of anti-endothelial cell IgA antibody in Behçet's disease.

Authors
 Sung Bin Cho  ;  Keun Jae Ahn  ;  Do Hee Kim  ;  Zhenlong Zheng  ;  Suhyun Cho  ;  Shin-Wook Kang  ;  Ju Hee Lee  ;  Yong-Beom Park  ;  Kwang Hoon Lee  ;  Dongsik Bang 
Citation
 Journal of Investigative Dermatology, Vol.132(3 pt 1) : 601-608, 2012 
Journal Title
 Journal of Investigative Dermatology 
ISSN
 0022-202X 
Issue Date
2012
MeSH
Adult ; Amino Acid Sequence ; Arthritis, Rheumatoid/blood ; Arthritis, Rheumatoid/immunology ; Autoantibodies/blood ; Autoantibodies/immunology* ; Autoantigens/immunology* ; Bacterial Proteins/immunology ; Behcet Syndrome/blood ; Behcet Syndrome/immunology* ; Female ; Glomerulonephritis, IGA/blood ; Glomerulonephritis, IGA/immunology ; Heterogeneous-Nuclear Ribonucleoprotein Group A-B/immunology* ; Humans ; Immunoglobulin A/blood ; Immunoglobulin A/immunology* ; Lupus Erythematosus, Systemic/blood ; Lupus Erythematosus, Systemic/immunology ; Male ; Middle Aged ; Molecular Sequence Data ; Streptococcus/immunology ; Takayasu Arteritis/blood ; Takayasu Arteritis/immunology
Keywords
Adult ; Amino Acid Sequence ; Arthritis, Rheumatoid/blood ; Arthritis, Rheumatoid/immunology ; Autoantibodies/blood ; Autoantibodies/immunology* ; Autoantigens/immunology* ; Bacterial Proteins/immunology ; Behcet Syndrome/blood ; Behcet Syndrome/immunology* ; Female ; Glomerulonephritis, IGA/blood ; Glomerulonephritis, IGA/immunology ; Heterogeneous-Nuclear Ribonucleoprotein Group A-B/immunology* ; Humans ; Immunoglobulin A/blood ; Immunoglobulin A/immunology* ; Lupus Erythematosus, Systemic/blood ; Lupus Erythematosus, Systemic/immunology ; Male ; Middle Aged ; Molecular Sequence Data ; Streptococcus/immunology ; Takayasu Arteritis/blood ; Takayasu Arteritis/immunology
Abstract
Behçet's disease (BD) is a chronic, multisystemic vasculitis that theoretically affects all sizes and types of blood vessels. Although pathogenesis remains enigmatic, endothelial cells are believed to be the primary target in this disease. We detected the target protein using western blotting and immunoprecipitation and determined the amino-acid sequence of the peptide by liquid chromatography-matrix assisted laser desorption/ionization-tandem time-of-flight analysis (LC-MALDI-TOF/TOF). Serum reactivity against the recombinant target protein was analyzed by immunoblotting. Serum reactivity against streptococcal 65-kD heat shock protein (hsp-65) and the recombinant target protein was investigated by ELISA. The 36-40-kD protein band that was obtained from immunoprecipitation, which was analyzed by LC-MALDI-TOF/TOF, exhibited the amino-acid sequences of heterogeneous nuclear ribonucleoproteins A2/B1 (hnRNP-A2/B1). Reactivity of serum IgA against human recombinant hnRNP-A2/B1 was detected in 25 of 30 BD patients (83.3%), 4 of 30 systemic lupus erythematosus patients (13.3%), 8 of 30 rheumatoid arthritis patients (26.7%), 9 of 30 Takayasu's arteritis patients (30%), 6 of 30 healthy controls (20%), and none of 30 IgA nephropathy patients. Optical densities obtained from ELISAs against the recombinant human hnRNP-A2/B1 were correlated with those against the recombinant streptococcal hsp-65.JID JOURNAL CLUB ARTICLE: For questions, answers, and open discussion about this article, please go to http://www.nature.com/jid/journalclub.
Full Text
http://www.nature.com/jid/journal/v132/n3-1/full/jid2011397a.html
DOI
22205302
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Dermatology (피부과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kang, Shin Wook(강신욱) ORCID logo https://orcid.org/0000-0002-5677-4756
Kim, Do Hee(김도희)
Park, Yong Beom(박용범)
Bang, Dong Sik(방동식)
Lee, Kwang Hoon(이광훈)
Lee, Ju Hee(이주희) ORCID logo https://orcid.org/0000-0002-1739-5956
Zheng, Zhen Long(정진룡)
Cho, Sung Bin(조성빈)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90869
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