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Maturation of periodontal tissues following implantation of rhGDF-5/β-TCP in one-wall intra-bony defects in dogs: 24-week histological observations

Authors
 Jung-Seok Lee  ;  Ulf M. E. Wikesjö  ;  Jung-Chul Park  ;  Yong-Ju Jang  ;  Susanne D. Pippig  ;  Patrizia Bastone  ;  Seong-Ho Choi  ;  Chong-Kwan Kim 
Citation
 JOURNAL OF CLINICAL PERIODONTOLOGY, Vol.39(5) : 466-474, 2012 
Journal Title
 JOURNAL OF CLINICAL PERIODONTOLOGY 
ISSN
 0303-6979 
Issue Date
2012
MeSH
Absorbable Implants ; Alveolar Bone Loss/pathology ; Alveolar Bone Loss/surgery* ; Alveolar Process/drug effects ; Alveolar Process/pathology ; Animals ; Biocompatible Materials* ; Bone Regeneration/drug effects ; Calcification, Physiologic/physiology ; Calcium Phosphates* ; Cell Count ; Collagen/drug effects ; Dental Cementum/drug effects ; Dental Cementum/pathology ; Dogs ; Epithelial Attachment/drug effects ; Epithelial Attachment/pathology ; Growth Differentiation Factor 5/therapeutic use* ; Guided Tissue Regeneration, Periodontal/methods* ; Humans ; Male ; Mandible/drug effects ; Mandible/pathology ; Osteocytes/drug effects ; Osteocytes/pathology ; Periodontal Ligament/drug effects ; Periodontal Ligament/pathology ; Random Allocation ; Recombinant Proteins ; Time Factors ; Tissue Engineering/methods ; Tissue Scaffolds*
Keywords
24 weeks ; dog ; maturation ; periodontal regeneration ; rhGDF-5 ; tissueengineering
Abstract
OBJECTIVE: Although a previous study reported that recombinant human growth/differentiation factor-5 (rhGDF-5) coated onto a β-tricalciumphosphate (β-TCP) significantly enhanced periodontal regeneration, the long-term stability/maturation of the regenerated tissues has not been demonstrated. The objective of this study was to evaluate periodontal regeneration/maturation following application of rhGDF-5/β-TCP using an established periodontal defect model and a 24-week healing interval. MATERIAL & METHODS: Unilateral, surgically created, 4 × 4 × 5 mm (length × width × height), one-wall, critical-size, intra-bony periodontal defects at the mandibular second and fourth premolar teeth in five young adult Beagle dogs received rhGDF-5/β-TCP. Bilateral sites at the fourth premolar in the other four dogs served as pristine controls receiving mucogingival flap surgery without defect induction. The animals were euthanized at 24 weeks for histological analysis. Unpublished data from the previous 8-week study were used to compare tissue maturation between 8 and 24 weeks. RESULTS: Linear histometric observations of cementum and alveolar regeneration showed no significant differences between the 8- and 24-week observation intervals. However, parameters of periodontal tissue maturation showed significant differences between the observation intervals including increased fraction mineralized tissue and lamellar bone (p < 0.05) and decreased osteocyte counts (p < 0.05) at 24 weeks compared with 8 weeks. Although the count inserting Sharpey's fibre did not significantly change, regenerated cementum remote from the intact periodontal ligament appeared more highly mineralized and thicker at 24 weeks compared with 8 weeks, and compared with the pristine cementum. Minimal β-TCP remained. CONCLUSIONS: These 24-week observations suggest that regenerated periodontal tissues in sites receiving rhGDF-5/β-TCP undergo progressive maturation without debilitating aberrant tissue reactions.
Full Text
http://onlinelibrary.wiley.com/doi/10.1111/j.1600-051X.2012.01862.x/abstract
DOI
22449372
Appears in Collections:
5. Research Institutes (연구소) > Research Center for Orofacial Hard Tissue Regeneration (구강악안면경조직재생연구센터) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Chong Kwan(김종관)
Park, Jung Chul(박정철)
Lee, Jung Seok(이중석) ORCID logo https://orcid.org/0000-0003-1276-5978
Choi, Seong Ho(최성호) ORCID logo https://orcid.org/0000-0001-6704-6124
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90704
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