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e novel expression of Oct3/4 and Bmi1 in the root development of mouse molars.

 Eizo Nakagawa  ;  Li Zhang  ;  Jeong-Oh Shin  ;  Eun-Jung Kim  ;  Sung-Won Cho  ;  Hayato Ohshima  ;  Zhi Chen  ;  Han-Sung Jung 
 CELL AND TISSUE RESEARCH, Vol.347(2) : 479-484, 2012 
Journal Title
Issue Date
Animals ; Mice ; Mice, Inbred ICR ; Molar/growth & development* ; Nuclear Proteins/metabolism* ; Odontoblasts/metabolism ; Odontogenesis ; Organic Cation Transport Proteins/metabolism* ; Polycomb Repressive Complex 1 ; Proto-Oncogene Proteins/metabolism* ; Repressor Proteins/metabolism* ; Tooth Root/growth & development* ; Tooth Root/metabolism
Tooth ; Root elongation ; Oct3/4 ; Bmi1 ; Stem cell
The root apex of the tooth elongates until the completion of root development. Although the signaling molecules inducing root elongation have been studied, the characteristic of the cells having the ability to maintain the root elongation remains unclear. This study aimed to investigate the characteristics of the cells involved in the root elongation. Octamer-binding factor 3/4 (Oct3/4) is known as one of the key regulators in maintaining the pluripotency and self-renewal properties of embryonic stem cells. Bmi1, the polycomb-group transcriptional repressor, has emerged as a key regulator in several cellular processes including stem cell self-renewal and cancer cell proliferation. At the beginning of root formation, ameloblasts expressed Oct3/4 in the nucleus, except in the apex of the cervical loop, in which Bmi1and cyclinD were expressed. At PN6, the expression of Oct3/4 in the ameloblasts shifted from the nucleus to the cytoplasm, whereas ameloblastin-negative Hertwig's epithelial root sheath (HERS) cells expressed Bmi1 and cyclinD. By PN10, the cells in the apex of HERS began to express Oct3/4 in their nucleus, whereas Bmi1 and cyclinD began to decrease in their expressions. The odontoblasts consistently expressed Oct3/4 in their cytoplasm. Our results suggest that (1) Oct3/4 creates the border between the ameloblasts from the proliferative region of HERS, (2) Bmi1-positive cells would be one of the candidates resulting in root elongation and (3) the Oct3/4 expression in the cytoplasm of odontoblasts may be related to maintain the odontoblastic characteristics.
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2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eun-Jung(김은정) ORCID logo https://orcid.org/0000-0002-9515-7590
Nakagawa, Eizo(나까가와에이죠)
Shin, Jeong Oh(신정오) ORCID logo https://orcid.org/0000-0002-6935-0936
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Cho, Sung Won(조성원) ORCID logo https://orcid.org/0000-0001-7505-9769
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