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The role of microRNA-23b in the differentiation of MSC into chondrocyte by targeting protein kinase A signaling

 Onju Ham  ;  Byeong-Wook Song  ;  Se-Yeon Lee  ;  Eunmi Choi  ;  Min-Ji Cha  ;  Chang Youn Lee  ;  Jun-Hee Park  ;  Il-Kwon Kim  ;  Woochul Chang  ;  Soyeon Limg  ;  Chang Hyun Lee  ;  Soonhag Kim  ;  Yangsoo Jang  ;  Ki-Chul Hwang 
 BIOMATERIALS, Vol.33(18) : 4500-4507, 2012 
Journal Title
Issue Date
Blotting, Western ; Cell Differentiation/drug effects ; Cell Differentiation/genetics ; Cells, Cultured ; Chondrocytes/cytology* ; Chondrocytes/metabolism* ; Cyclic AMP-Dependent Protein Kinases/genetics ; Cyclic AMP-Dependent Protein Kinases/metabolism* ; Humans ; Immunohistochemistry ; Isoquinolines/pharmacology ; MicroRNAs/genetics* ; MicroRNAs/physiology ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction ; Signal Transduction/drug effects ; Signal Transduction/genetics ; Sulfonamides/pharmacology
Chondrocyte ; Differentiation ; Mesenchymal stem cells ; MicroRNA ; PKA
Chondrogenic differentiation of mesenchymal stem cells (MSCs) is critical for successful cartilage regeneration. Several methods have been developed to attempt to chondrogenic differentiation, because chondrogenic differentiated cells can form stable cartilage and induce expression of a cartilage-specific phenotype. In this study, we found that both H-89 and microRNA-23b induced differentiation into chondrocyte of hMSCs through down-regulation of protein kinase A (PKA) signaling. The small molecule, H-89, was identified by PCA analysis as a potential mediator of chondrogenic differentiation. H-89 induced the expression of the chondrocyte marker, aggrecan, as well as miR-23b. We searched that miR-23b regulates protein level of PKA. When miR-23b was transfected into hMSCs, chondrogenic differentiation was induced. We confirmed the target of miR-23b using a reporter gene assay. Furthermore, not only H-89 or miR-23b-treated cells, but also cell co-treated with H-89 and miR-23b differentiated into chondrocytes. Our results indicate that H-89 induces the expression of endogenous miR-23b, thereby inducing chondrogenic differentiation by negatively inhibition of PKA signaling.
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1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Park, Jun-Hee(박준희)
Song, Byeong Wook(송병욱)
Lee, Se Yeon(이세연)
Lee, Chang Yeon(이창연)
Lim, So Yeon(임소연)
Jang, Yang Soo(장양수) ORCID logo https://orcid.org/0000-0002-2169-3112
Cha, Min Ji(차민지)
Choi, Eun Mi(최은미)
Ham, On Ju(함온주)
Hwang, Ki Chul(황기철)
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