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The role of microRNA-23b in the differentiation of MSC into chondrocyte by targeting protein kinase A signaling

DC Field Value Language
dc.contributor.author장양수-
dc.contributor.author차민지-
dc.contributor.author최은미-
dc.contributor.author함온주-
dc.contributor.author황기철-
dc.contributor.author박준희-
dc.contributor.author송병욱-
dc.contributor.author이세연-
dc.contributor.author이창연-
dc.contributor.author임소연-
dc.date.accessioned2014-12-19T16:32:52Z-
dc.date.available2014-12-19T16:32:52Z-
dc.date.issued2012-
dc.identifier.issn0142-9612-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/89736-
dc.description.abstractChondrogenic differentiation of mesenchymal stem cells (MSCs) is critical for successful cartilage regeneration. Several methods have been developed to attempt to chondrogenic differentiation, because chondrogenic differentiated cells can form stable cartilage and induce expression of a cartilage-specific phenotype. In this study, we found that both H-89 and microRNA-23b induced differentiation into chondrocyte of hMSCs through down-regulation of protein kinase A (PKA) signaling. The small molecule, H-89, was identified by PCA analysis as a potential mediator of chondrogenic differentiation. H-89 induced the expression of the chondrocyte marker, aggrecan, as well as miR-23b. We searched that miR-23b regulates protein level of PKA. When miR-23b was transfected into hMSCs, chondrogenic differentiation was induced. We confirmed the target of miR-23b using a reporter gene assay. Furthermore, not only H-89 or miR-23b-treated cells, but also cell co-treated with H-89 and miR-23b differentiated into chondrocytes. Our results indicate that H-89 induces the expression of endogenous miR-23b, thereby inducing chondrogenic differentiation by negatively inhibition of PKA signaling.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfBIOMATERIALS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCell Differentiation/drug effects-
dc.subject.MESHCell Differentiation/genetics-
dc.subject.MESHCells, Cultured-
dc.subject.MESHChondrocytes/cytology*-
dc.subject.MESHChondrocytes/metabolism*-
dc.subject.MESHCyclic AMP-Dependent Protein Kinases/genetics-
dc.subject.MESHCyclic AMP-Dependent Protein Kinases/metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHImmunohistochemistry-
dc.subject.MESHIsoquinolines/pharmacology-
dc.subject.MESHMicroRNAs/genetics*-
dc.subject.MESHMicroRNAs/physiology-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHSignal Transduction/drug effects-
dc.subject.MESHSignal Transduction/genetics-
dc.subject.MESHSulfonamides/pharmacology-
dc.titleThe role of microRNA-23b in the differentiation of MSC into chondrocyte by targeting protein kinase A signaling-
dc.typeArticle-
dc.contributor.collegeResearcher Institutes (부설 연구소)-
dc.contributor.departmentYonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단)-
dc.contributor.googleauthorOnju Ham-
dc.contributor.googleauthorByeong-Wook Song-
dc.contributor.googleauthorSe-Yeon Lee-
dc.contributor.googleauthorEunmi Choi-
dc.contributor.googleauthorMin-Ji Cha-
dc.contributor.googleauthorChang Youn Lee-
dc.contributor.googleauthorJun-Hee Park-
dc.contributor.googleauthorIl-Kwon Kim-
dc.contributor.googleauthorWoochul Chang-
dc.contributor.googleauthorSoyeon Limg-
dc.contributor.googleauthorChang Hyun Lee-
dc.contributor.googleauthorSoonhag Kim-
dc.contributor.googleauthorYangsoo Jang-
dc.contributor.googleauthorKi-Chul Hwang-
dc.identifier.doi22449550-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA03448-
dc.contributor.localIdA03995-
dc.contributor.localIdA04336-
dc.contributor.localIdA04456-
dc.contributor.localIdA01679-
dc.contributor.localIdA02026-
dc.contributor.localIdA02880-
dc.contributor.localIdA03244-
dc.contributor.localIdA04151-
dc.contributor.localIdA03373-1-
dc.relation.journalcodeJ00312-
dc.identifier.eissn1878-5905-
dc.identifier.pmid22449550-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0142961212002979-
dc.subject.keywordChondrocyte-
dc.subject.keywordDifferentiation-
dc.subject.keywordMesenchymal stem cells-
dc.subject.keywordMicroRNA-
dc.subject.keywordPKA-
dc.contributor.alternativeNameJang, Yang Soo-
dc.contributor.alternativeNameCha, Min Ji-
dc.contributor.alternativeNameChoi, Eun Mi-
dc.contributor.alternativeNameHam, On Ju-
dc.contributor.alternativeNameHwang, Ki Chul-
dc.contributor.alternativeNamePark, Jun-Hee-
dc.contributor.alternativeNameSong, Byeong Wook-
dc.contributor.alternativeNameLee, Se Yeon-
dc.contributor.alternativeNameLee, Chang Yeon-
dc.contributor.alternativeNameLim, So Yeon-
dc.contributor.affiliatedAuthorJang, Yang Soo-
dc.contributor.affiliatedAuthorCha, Min Ji-
dc.contributor.affiliatedAuthorHam, On Ju-
dc.contributor.affiliatedAuthorHwang, Ki Chul-
dc.contributor.affiliatedAuthorPark, Jun-Hee-
dc.contributor.affiliatedAuthorSong, Byeong Wook-
dc.contributor.affiliatedAuthorLee, Se Yeon-
dc.contributor.affiliatedAuthorLee, Chang Yeon-
dc.contributor.affiliatedAuthorChoi, Eun Mi-
dc.contributor.affiliatedAuthorLim, So Yeon-
dc.citation.volume33-
dc.citation.number18-
dc.citation.startPage4500-
dc.citation.endPage4507-
dc.identifier.bibliographicCitationBIOMATERIALS, Vol.33(18) : 4500-4507, 2012-
dc.identifier.rimsid31875-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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