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Consecutive targetable smart nanoprobe for molecular recognition of cytoplasmic microRNA in metastatic breast cancer

 Eunjung Kim  ;  Jaemoon Yang  ;  Joseph Park  ;  Soonhag Kim  ;  Nam Hee Kim  ;  Jong In Yook  ;  Jin-Suck Suh  ;  Seungjoo Haam  ;  Yong-Min Huh 
 ACS NANO, Vol.6(10) : 8525-8535, 2012 
Journal Title
Issue Date
Animals ; Breast Neoplasms/diagnosis* ; Breast Neoplasms/genetics ; Breast Neoplasms/secondary* ; Cell Line, Tumor ; Humans ; Mice ; MicroRNAs/analysis* ; MicroRNAs/genetics* ; Microscopy, Fluorescence/methods* ; Molecular Imaging/methods* ; Nanocapsules*/chemistry
hyaluronic acid ; intracellular imaging ; miR-34a ; molecular beacon ; nanocontainer
We report smart nanoprobe, hyaluronic acid (HA)-based nanocontainers containing miR-34a beacons (bHNCs), for the intracellular recognition of miR-34a levels in metastatic breast cancer cells, which is distinct from the imaging of biomarkers such of cell membrane receptors such as HER2. In this study, we demonstrate that a nanoscale vesicle that couples a targeting endocytic route, CD44, and a molecular imaging probe enables the efficient detection of specific miRNAs. Furthermore, bHNCs showed no cytotoxicity and high stability due to the anchored HA molecules on the surface of nanocontainers, and enables the targeted delivery of beacons via CD44 receptor-mediated endocytosis. In vitro and in vivo optical imaging using bHNCs also allow the measurement of miR-34a expression levels due to the selective recognition of the beacons released from the internalized bHNCs. We believe that the technique described herein can be further developed as a cancer diagnostic as well as a miRNA-based therapy of metastatic cancer.
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1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
5. Research Institutes (연구소) > Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단) > 1. Journal Papers
Yonsei Authors
Kim, Nam Hee(김남희) ORCID logo https://orcid.org/0000-0002-3087-5276
Suh, Jin Suck(서진석) ORCID logo https://orcid.org/0000-0001-9455-9240
Yang, Jae Moon(양재문) ORCID logo https://orcid.org/0000-0001-7365-0395
Yook, Jong In(육종인) ORCID logo https://orcid.org/0000-0002-7318-6112
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
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