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Organotypic hippocampal slice culture from the adult mouse brain: A versatile tool for translational neuropsychopharmacology

 Hyunjeong Kim  ;  Eosu Kim  ;  Minsun Park  ;  Eun Lee  ;  Kee Namkoong 
 Progress in Neuro-Psychopharmacology & Biological Psychiatry, Vol.41 : 36-43, 2013 
Journal Title
 Progress in Neuro-Psychopharmacology & Biological Psychiatry 
Issue Date
Age Factors ; Animals ; Brain/drug effects ; Brain/physiology ; Culture Media, Serum-Free/pharmacology ; Hippocampus/drug effects ; Hippocampus/physiology* ; Male ; Mice ; Mice, Inbred ICR ; Organ Culture Techniques/methods ; Psychopharmacology ; Translational Medical Research/methods*
Adult brain ; Hippocampus ; Mice ; Organotypic slice culture ; Serum-free
One of the most significant barriers towards translational neuropsychiatry would be an unavailability of living brain tissues. Although organotypic brain tissue culture could be a useful alternative enabling observation of temporal changes induced by various drugs in living brain tissues, a proper method to establish a stable organotypic brain slice culture system using adult (rather than neonatal) hippocampus has been still elusive. In this study, we evaluated our simple method using the serum-free culture medium for successful adult organotypic hippocampal slice culture. Several tens of hippocampal slices from a single adult mouse (3–5 months old) were cultured in serum-free versus serum-containing conventional culture medium for 30 days and underwent various experiments to validate the effects of the existence of serum in the culture medium. Neither the excessive regression of neuronal viability nor metabolic deficiency was observed in the serum-free medium culture in contrast to the serum-containing medium culture. Despite such viability, newly generated immature neurons were scarcely detected in the serum-free culture, suggesting that the original neurons in the brain slice persist rather than being replaced by neurogenesis. Key structural features of in vivo neural tissue constituting astrocytes, neural processes, and pre- and post-synapses were also well preserved in the serum-free culture. In conclusion, using the serum-free culture medium, the adult hippocampal slice culture system will serve as a promising ex vivo tool for various fields of neuroscience, especially for studies on aging-related neuropsychiatric disorders or for high throughput screening of potential agents working against such disorders.
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Appears in Collections:
5. Research Institutes (연구소) > Institute of Behavioral Sciences in Medicine (의학행동과학연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Psychiatry (정신과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Eosu(김어수) ORCID logo https://orcid.org/0000-0001-9472-9465
Kim, Hyun Jeong(김현정)
Namkoong, Kee(남궁기) ORCID logo https://orcid.org/0000-0003-1400-8057
Park, Min Sun(박민선)
Lee, Eun(이은) ORCID logo https://orcid.org/0000-0002-7462-0144
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