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Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment

Authors
 J.Y. Park  ;  Y.S. Yoon  ;  H.S. Park  ;  S.U. Kuh 
Citation
 Genetics and Molecular Research, Vol.12(1) : 838-851, 2013 
Journal Title
 Genetics and Molecular Research 
ISSN
 1676-5680 
Issue Date
2013
Abstract
We investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphatase, type I collagen, type II collagen, osteocalcin, and Sox9 in the 2 groups were compared by real-time PCR, before and following treatment with rhBMP-2 and TGF-β1. Immunoreactivity was analyzed to check protein activity. Type I collagen expression was significantly higher in cervical compared with that in lumbar disc cells. The mRNA expression was significantly increased after rhBMP-2 and TGF-β1 treatment. After rhBMP-2 treatment, mRNA expression of type I and II collagens increased significantly more in cervical than in lumbar NP cells. Following TGF-β1 treatment, the increase in mRNA expression was not significantly different between cervical and lumbar disc cells. Protein immunoreactivity, before and after cytokine treatment was similar to mRNA expression data. The matrix-related gene expression of cervical and lumbar NP after rhBMP-2 and TGF-β1 treatment increased similarly, with the exception of collagen expression.
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DOI
10.4238/2013.March.15.4
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
구성욱(Kuh, Sung Uk) ORCID logo https://orcid.org/0000-0003-2566-3209
박정윤(Park, Jeong Yoon) ORCID logo https://orcid.org/0000-0002-3728-7784
박효숙(Park, Hyo Suk)
윤영설(Yoon, Young Sul)
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87626
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