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Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment

 J.Y. Park  ;  Y.S. Yoon  ;  H.S. Park  ;  S.U. Kuh 
 GENETICS AND MOLECULAR RESEARCH , Vol.12(1) : 838-851, 2013 
Journal Title
Issue Date
Adult ; Aged ; Aggrecans/genetics ; Aggrecans/metabolism ; Alkaline Phosphatase/genetics ; Alkaline Phosphatase/metabolism ; Bone Morphogenetic Protein 2/pharmacology ; Cells, Cultured ; Cervical Vertebrae ; Collagen Type I/genetics ; Collagen Type I/metabolism ; Collagen Type II/genetics ; Collagen Type II/metabolism ; Cytokines/pharmacology* ; Fluorescent Antibody Technique ; Gene Expression Regulation/drug effects ; Humans ; Intervertebral Disc/drug effects* ; Intervertebral Disc/metabolism* ; Intervertebral Disc/pathology ; Intervertebral Disc Degeneration/genetics* ; Intervertebral Disc Degeneration/pathology ; Lumbar Vertebrae ; Microscopy, Fluorescence ; Middle Aged ; Osteocalcin/genetics ; Osteocalcin/metabolism ; Recombinant Proteins/pharmacology ; Reverse Transcriptase Polymerase Chain Reaction ; SOX9 Transcription Factor/genetics ; SOX9 Transcription Factor/metabolism ; Transforming Growth Factor beta/pharmacology ; Transforming Growth Factor beta1/pharmacology
Aggrecan ; Alkaline phosphatase ; Collagen ; Osteocalcin ; rhBMP-2 ; TGF-β1 ; TGF-β1
We investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphatase, type I collagen, type II collagen, osteocalcin, and Sox9 in the 2 groups were compared by real-time PCR, before and following treatment with rhBMP-2 and TGF-β1. Immunoreactivity was analyzed to check protein activity. Type I collagen expression was significantly higher in cervical compared with that in lumbar disc cells. The mRNA expression was significantly increased after rhBMP-2 and TGF-β1 treatment. After rhBMP-2 treatment, mRNA expression of type I and II collagens increased significantly more in cervical than in lumbar NP cells. Following TGF-β1 treatment, the increase in mRNA expression was not significantly different between cervical and lumbar disc cells. Protein immunoreactivity, before and after cytokine treatment was similar to mRNA expression data. The matrix-related gene expression of cervical and lumbar NP after rhBMP-2 and TGF-β1 treatment increased similarly, with the exception of collagen expression.
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1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kuh, Sung Uk(구성욱) ORCID logo https://orcid.org/0000-0003-2566-3209
Park, Jeong Yoon(박정윤) ORCID logo https://orcid.org/0000-0002-3728-7784
Park, Hyo Suk(박효숙)
Yoon, Young Sul(윤영설)
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