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Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment

DC FieldValueLanguage
dc.contributor.author구성욱-
dc.contributor.author박정윤-
dc.contributor.author박효숙-
dc.contributor.author윤영설-
dc.date.accessioned2014-12-18T09:09:00Z-
dc.date.available2014-12-18T09:09:00Z-
dc.date.issued2013-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/87626-
dc.description.abstractWe investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphatase, type I collagen, type II collagen, osteocalcin, and Sox9 in the 2 groups were compared by real-time PCR, before and following treatment with rhBMP-2 and TGF-β1. Immunoreactivity was analyzed to check protein activity. Type I collagen expression was significantly higher in cervical compared with that in lumbar disc cells. The mRNA expression was significantly increased after rhBMP-2 and TGF-β1 treatment. After rhBMP-2 treatment, mRNA expression of type I and II collagens increased significantly more in cervical than in lumbar NP cells. Following TGF-β1 treatment, the increase in mRNA expression was not significantly different between cervical and lumbar disc cells. Protein immunoreactivity, before and after cytokine treatment was similar to mRNA expression data. The matrix-related gene expression of cervical and lumbar NP after rhBMP-2 and TGF-β1 treatment increased similarly, with the exception of collagen expression.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfGENETICS AND MOLECULAR RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdult-
dc.subject.MESHAged-
dc.subject.MESHAggrecans/genetics-
dc.subject.MESHAggrecans/metabolism-
dc.subject.MESHAlkaline Phosphatase/genetics-
dc.subject.MESHAlkaline Phosphatase/metabolism-
dc.subject.MESHBone Morphogenetic Protein 2/pharmacology-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCervical Vertebrae-
dc.subject.MESHCollagen Type I/genetics-
dc.subject.MESHCollagen Type I/metabolism-
dc.subject.MESHCollagen Type II/genetics-
dc.subject.MESHCollagen Type II/metabolism-
dc.subject.MESHCytokines/pharmacology*-
dc.subject.MESHFluorescent Antibody Technique-
dc.subject.MESHGene Expression Regulation/drug effects-
dc.subject.MESHHumans-
dc.subject.MESHIntervertebral Disc/drug effects*-
dc.subject.MESHIntervertebral Disc/metabolism*-
dc.subject.MESHIntervertebral Disc/pathology-
dc.subject.MESHIntervertebral Disc Degeneration/genetics*-
dc.subject.MESHIntervertebral Disc Degeneration/pathology-
dc.subject.MESHLumbar Vertebrae-
dc.subject.MESHMicroscopy, Fluorescence-
dc.subject.MESHMiddle Aged-
dc.subject.MESHOsteocalcin/genetics-
dc.subject.MESHOsteocalcin/metabolism-
dc.subject.MESHRecombinant Proteins/pharmacology-
dc.subject.MESHReverse Transcriptase Polymerase Chain Reaction-
dc.subject.MESHSOX9 Transcription Factor/genetics-
dc.subject.MESHSOX9 Transcription Factor/metabolism-
dc.subject.MESHTransforming Growth Factor beta/pharmacology-
dc.subject.MESHTransforming Growth Factor beta1/pharmacology-
dc.titleMolecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Neurosurgery (신경외과학)-
dc.contributor.googleauthorJ.Y. Park-
dc.contributor.googleauthorY.S. Yoon-
dc.contributor.googleauthorH.S. Park-
dc.contributor.googleauthorS.U. Kuh-
dc.identifier.doi10.4238/2013.March.15.4-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00196-
dc.contributor.localIdA01650-
dc.contributor.localIdA01773-
dc.contributor.localIdA02578-
dc.relation.journalcodeJ00933-
dc.identifier.eissn1676-5680-
dc.identifier.pmid23546968-
dc.subject.keywordAggrecan-
dc.subject.keywordAlkaline phosphatase-
dc.subject.keywordCollagen-
dc.subject.keywordOsteocalcin-
dc.subject.keywordrhBMP-2-
dc.subject.keywordTGF-β1-
dc.subject.keywordTGF-β1-
dc.contributor.alternativeNameKuh, Sung Uk-
dc.contributor.alternativeNamePark, Jeong Yoon-
dc.contributor.alternativeNamePark, Hyo Suk-
dc.contributor.alternativeNameYoon, Young Sul-
dc.contributor.affiliatedAuthorKuh, Sung Uk-
dc.contributor.affiliatedAuthorPark, Jeong Yoon-
dc.contributor.affiliatedAuthorPark, Hyo Suk-
dc.contributor.affiliatedAuthorYoon, Young Sul-
dc.rights.accessRightsfree-
dc.citation.volume12-
dc.citation.number1-
dc.citation.startPage838-
dc.citation.endPage851-
dc.identifier.bibliographicCitationGENETICS AND MOLECULAR RESEARCH , Vol.12(1) : 838-851, 2013-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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