Cited 10 times in
Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment
DC Field | Value | Language |
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dc.contributor.author | 구성욱 | - |
dc.contributor.author | 박정윤 | - |
dc.contributor.author | 박효숙 | - |
dc.contributor.author | 윤영설 | - |
dc.date.accessioned | 2014-12-18T09:09:00Z | - |
dc.date.available | 2014-12-18T09:09:00Z | - |
dc.date.issued | 2013 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/87626 | - |
dc.description.abstract | We investigated the molecular response of degenerated human cervical and lumbar nucleus pulposus (NP) cells following cytokine treatment. Degenerated cervical and lumbar discs (8 each) were obtained from patients who underwent discectomy for degenerative disc disease; NP cells were isolated and cultured. The mRNA expressions of aggrecan, alkaline phosphatase, type I collagen, type II collagen, osteocalcin, and Sox9 in the 2 groups were compared by real-time PCR, before and following treatment with rhBMP-2 and TGF-β1. Immunoreactivity was analyzed to check protein activity. Type I collagen expression was significantly higher in cervical compared with that in lumbar disc cells. The mRNA expression was significantly increased after rhBMP-2 and TGF-β1 treatment. After rhBMP-2 treatment, mRNA expression of type I and II collagens increased significantly more in cervical than in lumbar NP cells. Following TGF-β1 treatment, the increase in mRNA expression was not significantly different between cervical and lumbar disc cells. Protein immunoreactivity, before and after cytokine treatment was similar to mRNA expression data. The matrix-related gene expression of cervical and lumbar NP after rhBMP-2 and TGF-β1 treatment increased similarly, with the exception of collagen expression. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | GENETICS AND MOLECULAR RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Aggrecans/genetics | - |
dc.subject.MESH | Aggrecans/metabolism | - |
dc.subject.MESH | Alkaline Phosphatase/genetics | - |
dc.subject.MESH | Alkaline Phosphatase/metabolism | - |
dc.subject.MESH | Bone Morphogenetic Protein 2/pharmacology | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Cervical Vertebrae | - |
dc.subject.MESH | Collagen Type I/genetics | - |
dc.subject.MESH | Collagen Type I/metabolism | - |
dc.subject.MESH | Collagen Type II/genetics | - |
dc.subject.MESH | Collagen Type II/metabolism | - |
dc.subject.MESH | Cytokines/pharmacology* | - |
dc.subject.MESH | Fluorescent Antibody Technique | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Intervertebral Disc/drug effects* | - |
dc.subject.MESH | Intervertebral Disc/metabolism* | - |
dc.subject.MESH | Intervertebral Disc/pathology | - |
dc.subject.MESH | Intervertebral Disc Degeneration/genetics* | - |
dc.subject.MESH | Intervertebral Disc Degeneration/pathology | - |
dc.subject.MESH | Lumbar Vertebrae | - |
dc.subject.MESH | Microscopy, Fluorescence | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Osteocalcin/genetics | - |
dc.subject.MESH | Osteocalcin/metabolism | - |
dc.subject.MESH | Recombinant Proteins/pharmacology | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | SOX9 Transcription Factor/genetics | - |
dc.subject.MESH | SOX9 Transcription Factor/metabolism | - |
dc.subject.MESH | Transforming Growth Factor beta/pharmacology | - |
dc.subject.MESH | Transforming Growth Factor beta1/pharmacology | - |
dc.title | Molecular response of human cervical and lumbar nucleus pulposus cells from degenerated discs following cytokine treatment | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Neurosurgery (신경외과학) | - |
dc.contributor.googleauthor | J.Y. Park | - |
dc.contributor.googleauthor | Y.S. Yoon | - |
dc.contributor.googleauthor | H.S. Park | - |
dc.contributor.googleauthor | S.U. Kuh | - |
dc.identifier.doi | 10.4238/2013.March.15.4 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00196 | - |
dc.contributor.localId | A01650 | - |
dc.contributor.localId | A01773 | - |
dc.contributor.localId | A02578 | - |
dc.relation.journalcode | J00933 | - |
dc.identifier.eissn | 1676-5680 | - |
dc.identifier.pmid | 23546968 | - |
dc.subject.keyword | Aggrecan | - |
dc.subject.keyword | Alkaline phosphatase | - |
dc.subject.keyword | Collagen | - |
dc.subject.keyword | Osteocalcin | - |
dc.subject.keyword | rhBMP-2 | - |
dc.subject.keyword | TGF-β1 | - |
dc.subject.keyword | TGF-β1 | - |
dc.contributor.alternativeName | Kuh, Sung Uk | - |
dc.contributor.alternativeName | Park, Jeong Yoon | - |
dc.contributor.alternativeName | Park, Hyo Suk | - |
dc.contributor.alternativeName | Yoon, Young Sul | - |
dc.contributor.affiliatedAuthor | Kuh, Sung Uk | - |
dc.contributor.affiliatedAuthor | Park, Jeong Yoon | - |
dc.contributor.affiliatedAuthor | Park, Hyo Suk | - |
dc.contributor.affiliatedAuthor | Yoon, Young Sul | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 12 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 838 | - |
dc.citation.endPage | 851 | - |
dc.identifier.bibliographicCitation | GENETICS AND MOLECULAR RESEARCH , Vol.12(1) : 838-851, 2013 | - |
dc.identifier.rimsid | 32171 | - |
dc.type.rims | ART | - |
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