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Development of a porcine skin injury model and characterization of the dose-dependent response to high-dose radiation

Authors
 Jun Won Kim  ;  Dong Won Lee  ;  Won Hoon Choi  ;  Yeo Reum Jeon  ;  Se Hoon Kim  ;  Haemi Cho  ;  Eun-Jung Lee  ;  Zhen-Yu Hong  ;  Won Jai Lee  ;  Jaeho Cho 
Citation
 JOURNAL OF RADIATION RESEARCH, Vol.54(5) : 823-831, 2013 
Journal Title
 JOURNAL OF RADIATION RESEARCH 
ISSN
 0449-3060 
Issue Date
2013
MeSH
Animals ; Disease Models, Animal* ; Dose-Response Relationship, Radiation ; Humans ; Radiation Injuries ; Radiodermatitis/etiology* ; Radiodermatitis/pathology* ; Radiodermatitis/physiopathology* ; Skin/pathology* ; Skin/physiopathology* ; Skin/radiation effects* ; Swine ; Swine, Miniature
Keywords
Animals ; Disease Models, Animal* ; Dose-Response Relationship, Radiation ; Humans ; Radiation Injuries ; Radiodermatitis/etiology* ; Radiodermatitis/pathology* ; Radiodermatitis/physiopathology* ; Skin/pathology* ; Skin/physiopathology* ; Skin/radiation effects* ; Swine ; Swine, Miniature
Abstract
A porcine skin model was developed to characterize the dose-dependent response to high-dose radiation. The dorsal skin of a mini pig was divided into four paraspinal sections, with 11 small irradiation fields (2 cm × 2 cm) in each section, and a single fraction of 15, 30, 50 or 75 Gy was delivered to each section using a 6 MeV electron beam. A spectrophotometer measured gross skin changes, and a biopsy for each radiation dose was performed in the 1st, 2nd, 4th, 6th and 9th weeks for histology, immunostaining with anti-CD31, and western blotting with IL-6 and TGF-β1 to determine the degree of skin damage. After a 4-week latency period, erythema and dry desquamation, moist desquamation, and ulceration appeared at 4, 6 and 9 weeks, respectively. Gross skin toxicity was more pronounced, occurred early and continued to progress with irradiation >50 Gy, whereas complete healing was observed 12 weeks after 15 Gy. Spectrophotometry showed erythema indices rapidly increased during the first 4 weeks after irradiation. The number of eosinophils began rising sharply at 4 weeks and normalized after reaching peaks at 7–8 weeks. Microvessel density showed a biphasic pattern with a transient peak at 1 week, a nadir at 4–6 weeks, and maximum recovery at 9 weeks. Increase in the levels of IL-6 and TGF-β1 was detected soon after irradiation. Most of these parameters indicated complete healing of the skin 12 weeks after 15 Gy. Our porcine skin model provides an effective platform for studying high-dose radiation-induced skin injury, in particular histologic and molecular changes, during the early latency period.
Files in This Item:
T201302759.pdf Download
DOI
10.1093/jrr/rrt016
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Plastic and Reconstructive Surgery (성형외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Kim, Jun Won(김준원) ORCID logo https://orcid.org/0000-0003-1358-364X
Lee, Dong Won(이동원) ORCID logo https://orcid.org/0000-0003-0046-3139
Lee, Won Jai(이원재) ORCID logo https://orcid.org/0000-0003-3056-0503
Lee, Eun Jung(이은정)
Cho, Jae Ho(조재호) ORCID logo https://orcid.org/0000-0001-9966-5157
Cho, Hae Mi(조해미)
Choi, Won Hoon(최원훈)
Hong, Zhen-Yu(홍진우)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87585
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