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Two Distinct Functional Patterns of Hepatitis C Virus (HCV)-Specific T Cell Responses in Seronegative, Aviremic Patients

Authors
 Yoon Seok Choi  ;  Jung Eun Lee  ;  Seung Joo Nam  ;  Jung Tak Park  ;  Hyon-Suk Kim  ;  Kyu Hun Choi  ;  Beom Seok Kim  ;  Eui-Cheol Shin 
Citation
 PLOS ONE, Vol.8(4) : e62319, 2013 
Journal Title
 PLOS ONE 
Issue Date
2013
MeSH
Aged ; Cytokines/analysis ; Cytokines/immunology ; Female ; HIV Seronegativity* ; Hepacivirus/immunology* ; Hepatitis C/blood ; Hepatitis C/immunology* ; Humans ; Male ; Middle Aged ; Renal Dialysis ; T-Lymphocytes/immunology* ; T-Lymphocytes/virology
Keywords
Aged ; Cytokines/analysis ; Cytokines/immunology ; Female ; HIV Seronegativity* ; Hepacivirus/immunology* ; Hepatitis C/blood ; Hepatitis C/immunology* ; Humans ; Male ; Middle Aged ; Renal Dialysis ; T-Lymphocytes/immunology* ; T-Lymphocytes/virology
Abstract
In hepatitis C Virus (HCV) high-risk groups, HCV-specific T cell responses have been detected in seronegative, aviremic persons who have no evidence of HCV infection. Herein, we investigated functional profiles of HCV-specific T-cell responses in seronegative, aviremic patients of a HCV high-risk group. Seventy seven hemodialysis patients with chronic renal disease were analyzed by IFN-γ ELISpot assays, and eight of 71 (11.3%) seronegative, aviremic patients displayed HCV-specific T-cell responses. Their HCV-specific memory T cells were characterized by assessing cytokine polyfunctionality, known to provide antiviral protection. By intracellular staining of IFN-γ, TNF-α, IL-2 and MIP-1β, we identified two distinct populations in the seronegative, aviremic patients: polyfunctional responders and TNF-α-predominant responders. In further analysis, occult HCV infection was excluded as a cause of the HCV-specific T cell response via secondary nested RT-PCR of HCV RNA in peripheral blood mononuclear cell samples. HCV-specific T cells targeted multiple epitopes including non-structural proteins in a single patient, implying that their T cells might have been primed by HCV proteins synthesized within the host. We conclude that HCV-specific memory T cells of seronegative, aviremic patients arise from authentic HCV replication in the host, but not from current occult HCV infection. By functional pattern of HCV-specific T cells, there are two distinct populations in these patients: polyfunctional responders and TNF-α-predominant responders.
Files in This Item:
T201302461.pdf Download
DOI
10.1371/journal.pone.0062319
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Beom Seok(김범석) ORCID logo https://orcid.org/0000-0002-5732-2583
Kim, Hyon Suk(김현숙) ORCID logo https://orcid.org/0000-0001-5662-7740
Park, Jung Tak(박정탁) ORCID logo https://orcid.org/0000-0002-2325-8982
Lee, Jung Eun(이정은) ORCID logo https://orcid.org/0000-0003-0917-2872
Choi, Kyu Hun(최규헌) ORCID logo https://orcid.org/0000-0003-0095-9011
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/87390
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