Cited 6 times in
Two Distinct Functional Patterns of Hepatitis C Virus (HCV)-Specific T Cell Responses in Seronegative, Aviremic Patients
DC Field | Value | Language |
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dc.contributor.author | 김범석 | - |
dc.contributor.author | 김현숙 | - |
dc.contributor.author | 박정탁 | - |
dc.contributor.author | 이정은 | - |
dc.contributor.author | 최규헌 | - |
dc.date.accessioned | 2014-12-18T09:01:32Z | - |
dc.date.available | 2014-12-18T09:01:32Z | - |
dc.date.issued | 2013 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/87390 | - |
dc.description.abstract | In hepatitis C Virus (HCV) high-risk groups, HCV-specific T cell responses have been detected in seronegative, aviremic persons who have no evidence of HCV infection. Herein, we investigated functional profiles of HCV-specific T-cell responses in seronegative, aviremic patients of a HCV high-risk group. Seventy seven hemodialysis patients with chronic renal disease were analyzed by IFN-γ ELISpot assays, and eight of 71 (11.3%) seronegative, aviremic patients displayed HCV-specific T-cell responses. Their HCV-specific memory T cells were characterized by assessing cytokine polyfunctionality, known to provide antiviral protection. By intracellular staining of IFN-γ, TNF-α, IL-2 and MIP-1β, we identified two distinct populations in the seronegative, aviremic patients: polyfunctional responders and TNF-α-predominant responders. In further analysis, occult HCV infection was excluded as a cause of the HCV-specific T cell response via secondary nested RT-PCR of HCV RNA in peripheral blood mononuclear cell samples. HCV-specific T cells targeted multiple epitopes including non-structural proteins in a single patient, implying that their T cells might have been primed by HCV proteins synthesized within the host. We conclude that HCV-specific memory T cells of seronegative, aviremic patients arise from authentic HCV replication in the host, but not from current occult HCV infection. By functional pattern of HCV-specific T cells, there are two distinct populations in these patients: polyfunctional responders and TNF-α-predominant responders. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Cytokines/analysis | - |
dc.subject.MESH | Cytokines/immunology | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | HIV Seronegativity* | - |
dc.subject.MESH | Hepacivirus/immunology* | - |
dc.subject.MESH | Hepatitis C/blood | - |
dc.subject.MESH | Hepatitis C/immunology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Renal Dialysis | - |
dc.subject.MESH | T-Lymphocytes/immunology* | - |
dc.subject.MESH | T-Lymphocytes/virology | - |
dc.title | Two Distinct Functional Patterns of Hepatitis C Virus (HCV)-Specific T Cell Responses in Seronegative, Aviremic Patients | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Yoon Seok Choi | - |
dc.contributor.googleauthor | Jung Eun Lee | - |
dc.contributor.googleauthor | Seung Joo Nam | - |
dc.contributor.googleauthor | Jung Tak Park | - |
dc.contributor.googleauthor | Hyon-Suk Kim | - |
dc.contributor.googleauthor | Kyu Hun Choi | - |
dc.contributor.googleauthor | Beom Seok Kim | - |
dc.contributor.googleauthor | Eui-Cheol Shin | - |
dc.identifier.doi | 10.1371/journal.pone.0062319 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00488 | - |
dc.contributor.localId | A01654 | - |
dc.contributor.localId | A04043 | - |
dc.contributor.localId | A03119 | - |
dc.contributor.localId | A01117 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 23638039 | - |
dc.subject.keyword | Aged | - |
dc.subject.keyword | Cytokines/analysis | - |
dc.subject.keyword | Cytokines/immunology | - |
dc.subject.keyword | Female | - |
dc.subject.keyword | HIV Seronegativity* | - |
dc.subject.keyword | Hepacivirus/immunology* | - |
dc.subject.keyword | Hepatitis C/blood | - |
dc.subject.keyword | Hepatitis C/immunology* | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Male | - |
dc.subject.keyword | Middle Aged | - |
dc.subject.keyword | Renal Dialysis | - |
dc.subject.keyword | T-Lymphocytes/immunology* | - |
dc.subject.keyword | T-Lymphocytes/virology | - |
dc.contributor.alternativeName | Kim, Beom Seok | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.alternativeName | Park, Jung Tak | - |
dc.contributor.alternativeName | Lee, Jung Eun | - |
dc.contributor.alternativeName | Choi, Kyu Hun | - |
dc.contributor.affiliatedAuthor | Kim, Beom Seok | - |
dc.contributor.affiliatedAuthor | Park, Jung Tak | - |
dc.contributor.affiliatedAuthor | Choi, Kyu Hun | - |
dc.contributor.affiliatedAuthor | Lee, Jung Eun | - |
dc.contributor.affiliatedAuthor | Kim, Hyon Suk | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 8 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | e62319 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.8(4) : e62319, 2013 | - |
dc.identifier.rimsid | 33025 | - |
dc.type.rims | ART | - |
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