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Identification of Novel Allergenic Components from German Cockroach Fecal Extract by a Proteomic Approach

Authors
 Jeong K.Y.  ;  Kim C.  ;  Park J.  ;  Han I.-S.  ;  Park J.-W.  ;  Yong T.-S. 
Citation
 INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY, Vol.161(4) : 315-324, 2013 
Journal Title
 INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY 
ISSN
 1018-2438 
Issue Date
2013
MeSH
Adolescent ; Adult ; Allergens/genetics ; Allergens/immunology ; Allergens/isolation & purification ; Allergens/metabolism* ; Amino Acid Sequence ; Animals ; Blattellidae/immunology* ; Child ; Child, Preschool ; Feces/chemistry ; Female ; Humans ; Hypersensitivity ; Immunoglobulin E/blood ; Immunoglobulin E/immunology ; Insect Proteins/immunology* ; Insect Proteins/isolation & purification ; Male ; Middle Aged ; Molecular Sequence Data ; Sequence Alignment ; Transgenes/genetics ; Young Adult ; alpha-Amylases/genetics ; alpha-Amylases/immunology ; alpha-Amylases/isolation & purification ; alpha-Amylases/metabolism*
Keywords
α-Amylase ; Allergen ; Cockroach
Abstract
BACKGROUND: Cockroaches produce potent allergens, and cockroach feces are known to be especially rich in allergens. In this study, we analyze the allergenic components from cockroach feces and evaluate allergenicity of recombinant α-amylase identified from fecal extract. METHODS: IgE-reactive proteins from German cockroach fecal extract were analyzed by proteomic analysis and immunoblotting. Recombinant α-amylase was produced and its allergenicity was evaluated by ELISA. RESULTS: Analysis of German cockroach fecal extracts identified 12 IgE-reactive components. Most of these allergens were found to be digestive enzymes such as α-amylase, trypsin, chymotrypsin, metalloprotease, and midgut carboxypeptidase A, but the identity of 3 IgE-reactive proteins is still unknown. Glycinin-like proteins, which were likely derived from the cockroach diet, were also identified. German cockroach α-amylase shares the highest identity with pig α-amylase (55.8%), followed by mite group 4 allergens (Blo t 4, 50.4%; Der p 4, 49.8%; Eur m 4, 47.4%). In this study, recombinant α-amylase from German cockroach was expressed, and its allergenicity was examined by ELISA. Specific IgE against recombinant amylase was detected in 41.4% (12/29) of serum samples from German cockroach-sensitized subjects. Recombinant α-amylase was able to inhibit 55% of specific IgE to German cockroach whole-body extract. CONCLUSIONS: Amylase was found to be an important novel allergen in cockroach feces. It is hoped that recombinant α-amylase will be useful for further studies and clinical applications.
Full Text
http://www.karger.com/Article/FullText/347034
DOI
10.1159/000347034
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
5. Research Institutes (연구소) > Institute of Allergy (알레르기연구소) > 1. Journal Papers
Yonsei Authors
Kim, Chung Ryul(김충렬)
Park, Jung Won(박중원) ORCID logo https://orcid.org/0000-0003-0249-8749
Park, Ji Na(박지나)
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Jeong, Kyoung Yong(정경용) ORCID logo https://orcid.org/0000-0001-9887-1426
Han In Soo(한인수)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/86952
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