Sleeping Beauty transposon system harboring HRAS, c-Myc and shp53 induces sarcomatoid carcinomas in mouse skin

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Authors: SUNYOUNG JUNG ; SIMON WEONSANG RO ; GEUNYOUNG JUNG ; HYE-LIM JU ; EUN-SIL YU ; WOO-CHAN SON

MeSH: Animals ; Carcinoma/diagnostic imaging ; Carcinoma/pathology* ; Cell Transformation, Neoplastic/genetics ; DNA Transposable Elements/genetics* ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Injections, Subcutaneous ; Mice ; Mice, Inbred C57BL ; Mutagenesis, Insertional/genetics ; Positron-Emission Tomography ; Proto-Oncogene Proteins c-myc/administration & dosage ; Proto-Oncogene Proteins c-myc/genetics* ; Proto-Oncogene Proteins p21(ras)/administration & dosage ; Proto-Oncogene Proteins p21(ras)/genetics* ; RNA, Small Interfering/administration & dosage ; RNA, Small Interfering/genetics ; Radiography ; Transposases/genetics* ; Tumor Suppressor Protein p53/genetics*

Keywords: Animals ; Carcinoma/diagnostic imaging ; Carcinoma/pathology* ; Cell Transformation, Neoplastic/genetics ; DNA Transposable Elements/genetics* ; Female ; Gene Expression Regulation, Neoplastic ; Humans ; Injections, Subcutaneous ; Mice ; Mice, Inbred C57BL ; Mutagenesis, Insertional/genetics ; Positron-Emission Tomography ; Proto-Oncogene Proteins c-myc/administration & dosage ; Proto-Oncogene Proteins c-myc/genetics* ; Proto-Oncogene Proteins p21(ras)/administration & dosage ; Proto-Oncogene Proteins p21(ras)/genetics* ; RNA, Small Interfering/administration & dosage ; RNA, Small Interfering/genetics ; Radiography ; Transposases/genetics* ; Tumor Suppressor Protein p53/genetics*

Abstract: The Sleeping Beauty transposon system is used as a tool for insertional mutagenesis and oncogenesis. However, little is known about the exact histological phenotype of the tumors induced. Thus, we used immunohistochemical markers to enable histological identification of the type of tumor induced by subcutaneous injection of the HRAS, c-Myc and shp53 oncogenes in female C57BL/6 mice. The tumor was removed when it reached 100 mm3 in volume. Subsequently, we used 13 immunohistochemical markers to histologically identify the tumor type. The results suggested that the morphology of the tumor was similar to that of sarcomatoid carcinoma.