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Preparation and Characterization of an Extract of German Cockroach From a Korean Source

Authors
 Kyoung Yong Jeong ; Soo-Young Choi ; Jung-Won Park ; Chein-Soo Hong ; Tai-Soon Yong ; Joo-Shil Lee ; Jae-Hyun Lee 
Citation
 Allergy Asthma and Immunology Research, Vol.5(2) : 102~105, 2013 
Journal Title
 Allergy Asthma and Immunology Research 
ISSN
 2092-7355 
Issue Date
2013
Abstract
PURPOSE: The cockroach (CR) is an important cause of respiratory allergic disorders. We prepared a German CR extract in a standardized way and analyzed its allergenic properties. METHODS: The extract was prepared from German CR (Blattella germanica) obtained from a Korean colony, and its allergenic activity was compared with that of the commercial Hollister-Stier (HS) extract. The concentrations of Bla g 1 and Bla g 2 were measured, and an in vitro specific IgE binding inhibition assay was performed to assess IgE reactivity. Proteolytic activity was examined by gelatin zymography. RESULTS: Bla g 1 and Bla g 2 were detected at 405 U/mg and 273 ng/mg, respectively, in the Korean extract, and at 187 U/mg and 56 ng/mg, respectively, in the HS extract. The Korean extract showed 94.2% inhibition of IgE reactivity, as compared with the HS extract. A similar pattern of IgE-reactive bands was detected for the two extracts, indicating that their allergenic components are similar. The proteolytic activities of the Korean and HS extracts were found to be similar in gelatin zymography. The endotoxin levels in the Korean and HS extracts were 3,440 EU/mL and 6,580 EU/mL, respectively. CONCLUSIONS: The German CR extract was prepared in a standardized way. The extract produced in this study will be useful for the development of allergy diagnostics and immunotherapeutic agents
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/86711
DOI
10.4168/aair.2013.5.2.102
Appears in Collections:
1. 연구논문 > 5. Research Institutes > Institute of Allergy
1. 연구논문 > 1. College of Medicine > Dept. of Internal Medicine
1. 연구논문 > 1. College of Medicine > Dept. of Environmental Medical Biology
1. 연구논문 > 1. College of Medicine > Medical Research Center
Yonsei Authors
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