Cited 7 times in
Preparation and Characterization of an Extract of German Cockroach From a Korean Source
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박중원 | - |
dc.contributor.author | 용태순 | - |
dc.contributor.author | 이재현 | - |
dc.contributor.author | 정경용 | - |
dc.contributor.author | 최수영 | - |
dc.date.accessioned | 2014-12-18T08:39:22Z | - |
dc.date.available | 2014-12-18T08:39:22Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 2092-7355 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/86711 | - |
dc.description.abstract | PURPOSE: The cockroach (CR) is an important cause of respiratory allergic disorders. We prepared a German CR extract in a standardized way and analyzed its allergenic properties. METHODS: The extract was prepared from German CR (Blattella germanica) obtained from a Korean colony, and its allergenic activity was compared with that of the commercial Hollister-Stier (HS) extract. The concentrations of Bla g 1 and Bla g 2 were measured, and an in vitro specific IgE binding inhibition assay was performed to assess IgE reactivity. Proteolytic activity was examined by gelatin zymography. RESULTS: Bla g 1 and Bla g 2 were detected at 405 U/mg and 273 ng/mg, respectively, in the Korean extract, and at 187 U/mg and 56 ng/mg, respectively, in the HS extract. The Korean extract showed 94.2% inhibition of IgE reactivity, as compared with the HS extract. A similar pattern of IgE-reactive bands was detected for the two extracts, indicating that their allergenic components are similar. The proteolytic activities of the Korean and HS extracts were found to be similar in gelatin zymography. The endotoxin levels in the Korean and HS extracts were 3,440 EU/mL and 6,580 EU/mL, respectively. CONCLUSIONS: The German CR extract was prepared in a standardized way. The extract produced in this study will be useful for the development of allergy diagnostics and immunotherapeutic agents | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | ALLERGY ASTHMA & IMMUNOLOGY RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Preparation and Characterization of an Extract of German Cockroach From a Korean Source | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Institute of Allergy (알레르기연구소) | - |
dc.contributor.googleauthor | Kyoung Yong Jeong | - |
dc.contributor.googleauthor | Soo-Young Choi | - |
dc.contributor.googleauthor | Jae-Hyun Lee | - |
dc.contributor.googleauthor | Joo-Shil Lee | - |
dc.contributor.googleauthor | Tai-Soon Yong | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.contributor.googleauthor | Jung-Won Park | - |
dc.identifier.doi | 10.4168/aair.2013.5.2.102 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A02424 | - |
dc.contributor.localId | A03086 | - |
dc.contributor.localId | A03572 | - |
dc.contributor.localId | A04093 | - |
dc.relation.journalcode | J00064 | - |
dc.identifier.eissn | 2092-7363 | - |
dc.identifier.pmid | 23449858 | - |
dc.subject.keyword | Allergen | - |
dc.subject.keyword | German cockroach | - |
dc.subject.keyword | Korea | - |
dc.subject.keyword | standardization | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.alternativeName | Lee, Jae Hyun | - |
dc.contributor.alternativeName | Jeong, Kyoung Yong | - |
dc.contributor.alternativeName | Choi, Soo Young | - |
dc.contributor.affiliatedAuthor | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Lee, Jae Hyun | - |
dc.contributor.affiliatedAuthor | Jeong, Kyoung Yong | - |
dc.contributor.affiliatedAuthor | Choi, Soo Young | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 5 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 102 | - |
dc.citation.endPage | 105 | - |
dc.identifier.bibliographicCitation | ALLERGY ASTHMA & IMMUNOLOGY RESEARCH, Vol.5(2) : 102-105, 2013 | - |
dc.identifier.rimsid | 29155 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.