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Existence of glioma stroma mesenchymal stemlike cells in Korean glioma specimens

 Young Goo Kim  ;  Soyoun Jeon  ;  Ga-Yeong Sin  ;  Jin-Kyoung Shim  ;  Bo-Kyung Kim  ;  Hye-Jin Shin  ;  Ji-Hyun Lee  ;  Yong-Min Huh  ;  Su-Jae Lee  ;  Eui-Hyun Kim  ;  Eun Kyung Park  ;  Se-Hoon Kim  ;  Jong Hee Chang  ;  Dong Seok Kim  ;  Sun Ho Kim  ;  Yong-Kil Hong  ;  Seok-Gu Kang  ;  Frederick F. Lang 
 CHILDS NERVOUS SYSTEM, Vol.29(4) : 549-563, 2013 
Journal Title
Issue Date
Animals ; Antigens, CD/metabolism ; Brain Neoplasms/metabolism ; Brain Neoplasms/pathology* ; Cell Separation ; Flow Cytometry ; Glioma/metabolism ; Glioma/pathology* ; Humans ; Male ; Mesenchymal Stromal Cells/metabolism ; Mesenchymal Stromal Cells/pathology* ; Mice ; Mice, Nude ; Republic of Korea
CD 105 ; Glioma stroma ; Mesenchymal stemlike cells ; Microenvironment ; Perivascular area
PURPOSE: It was presented that mesenchymal stem cells (MSCs) can be isolated from western glioma specimens. However, whether MSCs exist in glioma specimens of different ethnicities is unknown. To verify the existence of MSCs in an independent cohort, we undertook studies to isolate MSCs from a group of Korean patients. We hypothesized that cells resembling MSCs that were deemed mesenchymal stemlike cells (MSLCs) exist in an independent cohort of Korean gliomas. METHODS: We cultured fresh glioma specimens using the protocols used for culturing MSCs. The cultured cells were analyzed with fluorescence-activated cell sorting (FACS) for surface markers associated with MSCs. Cultured cells were exposed to mesenchymal differentiation conditions. To presume possible locations of MSLCs in the glioma, sections of glioma were analyzed by immunofluorescent labeling for CD105, CD31, and NG2. RESULTS: From nine of 31 glioma specimens, we isolated cells resembling MSCs, which were deemed Korean glioma stroma MSLCs (KGS-MSLCs). KGS-MSLCs were spindle shaped and adherent to plastic. KGS-MSLCs had similar surface markers to MSCs (CD105(+), CD90(+), CD73(+), and CD45(-)). KGS-MSLCs were capable of mesenchymal differentiation and might be located around endothelial cells, pericytes, and in a disorganized perivascular area inside glioma stroma. CONCLUSIONS: We found that cells resembling MSCs indeed exist in an independent cohort of glioma patients, as presented in western populations. We could presume that the possible location of KGS-MSLCs was in perivascular area or in glioma stroma that was a disorganized vascular niche. It might be possible that KGS-MSLCs could be one of constituent of stroma of glioma microenvironment.
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1. College of Medicine (의과대학) > Dept. of Neurosurgery (신경외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
Yonsei Authors
Kang, Seok Gu(강석구) ORCID logo https://orcid.org/0000-0001-5676-2037
Kim, Dong Seok(김동석)
Kim, Sun Ho(김선호) ORCID logo https://orcid.org/0000-0003-0970-3848
Kim, Se Hoon(김세훈) ORCID logo https://orcid.org/0000-0001-7516-7372
Kim, Eui Hyun(김의현) ORCID logo https://orcid.org/0000-0002-2523-7122
Park, Eun Kyung(박은경)
Lee, Ji Hyun(이지현) ORCID logo https://orcid.org/0000-0002-9223-9478
Chang, Jong Hee(장종희)
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
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