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Effect of anti-sclerostin antibody on orthodontic tooth movement in ovariectomized rats

Authors
 Hyunna Ahn  ;  Wonse Park  ;  Sung-Hwan Choi  ;  Namki Hong  ;  Jisun Huh  ;  Seoyeon Jung 
Citation
 PROGRESS IN ORTHODONTICS, Vol.25(1) : 45, 2024-11 
Journal Title
PROGRESS IN ORTHODONTICS
ISSN
 1723-7785 
Issue Date
2024-11
MeSH
Alveolar Process* / diagnostic imaging ; Alveolar Process* / drug effects ; Alveolar Process* / pathology ; Animals ; Antibodies, Monoclonal / pharmacology ; Antibodies, Monoclonal / therapeutic use ; Bone Morphogenetic Proteins ; Female ; Genetic Markers ; Osteoclasts / drug effects ; Ovariectomy* ; Random Allocation ; Rats ; Rats, Sprague-Dawley* ; Tartrate-Resistant Acid Phosphatase ; Tooth Movement Techniques* / instrumentation ; Tooth Movement Techniques* / methods ; X-Ray Microtomography*
Keywords
Bone biology ; Bone remodeling ; Orthodontics ; Osteogenesis ; Osteoporosis ; Wnt signaling pathway
Abstract
Background: This study aimed to evaluate the effect of systemically administered anti-sclerostin antibodies (Anti-Scl Ab) on orthodontic tooth movements (OTM) in an ovariectomized rat.

Methods: Twenty-four 12-week-old female Sprague-Dawley rats were randomly divided into two groups: (1) ovariectomy (OVX) group, (2) ovariectomy + romosozumab (ROMO) group. OTM was performed 8 weeks after OVX. The ROMO group received subcutaneous injections of romosozumab twice a week, starting two weeks after OVX. Eight weeks after the OVX, an orthodontic force of 50 g was measured and applied by connecting orthodontic elastic bands between the maxillary first molar and a mini-screw to facilitate tooth movement (orthodontic treatment). Subsequently, the three rats were sacrificed on days 5, 7, 10, and 14. The plaster models were scanned to measure the amount of tooth movement. The effects on alveolar bone and periodontal tissues were evaluated through micro-computed tomography (micro-CT) analysis, tartrate-resistant acid phosphatase (TRAP) staining, and immunohistochemistry (IHC) analysis.

Results: The ROMO group showed more tooth movement on day 7 of orthodontic treatment. Conversely, on days 10 and 14, relatively less movement was observed. Analysis of the root furcation area of the maxillary first molars revealed that from the 7th day, BV/TV, Tb.N., Tb.Th. increased, while Tb.Sp. decreased in the ROMO group. More TRAP-positive cells were observed in the compression side of the OVX group, the ROMO group exhibited a marked decrease in the positive expression of the RANKL, OPG, and sclerostin. The OPG/RANKL ratio showed significant differences in expression between the two groups. The ROMO group exhibited a higher OPG/RANKL ratio than the OVX group, and the tension side exhibited a higher OPG/RANKL ratio demonstrating significant differences.

Conclusion: Romosozumab initially accelerated tooth movements, but later decreased tooth movement. As new alveolar bone is formed, the micro-CT parameters are also improved. Osteoclasts, RANKL, OPG, and sclerostin decreased, while the OPG/RANKL ratio became higher.
Files in This Item:
T992024876.pdf Download
DOI
10.1186/s40510-024-00544-0
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Dental Education (치의학교육학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Advanced General Dentistry (통합치의학과) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
Yonsei Authors
Park, Wonse(박원서) ORCID logo https://orcid.org/0000-0002-2081-1156
Jung, Seoyeon(정서연)
Choi, Sung Hwan(최성환) ORCID logo https://orcid.org/0000-0002-1150-0268
Huh, Jisun(허지선) ORCID logo https://orcid.org/0000-0001-5514-187X
Hong, Nam Ki(홍남기) ORCID logo https://orcid.org/0000-0002-8246-1956
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/201755
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