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Ptychographic lens-less birefringence microscopy using a mask-modulated polarization image sensor

 Jeongsoo Kim  ;  Seungri Song  ;  Hongseong Kim  ;  Bora Kim  ;  Mirae Park  ;  Seung Jae Oh  ;  Daesuk Kim  ;  Barry Cense  ;  Yong-Min Huh  ;  Joo Yong Lee  ;  Chulmin Joo 
 SCIENTIFIC REPORTS, Vol.13(1) : 19263, 2023-11 
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Birefringence, an inherent characteristic of optically anisotropic materials, is widely utilized in various imaging applications ranging from material characterizations to clinical diagnosis. Polarized light microscopy enables high-resolution, high-contrast imaging of optically anisotropic specimens, but it is associated with mechanical rotations of polarizer/analyzer and relatively complex optical designs. Here, we present a form of lens-less polarization-sensitive microscopy capable of complex and birefringence imaging of transparent objects without an optical lens and any moving parts. Our method exploits an optical mask-modulated polarization image sensor and single-input-state LED illumination design to obtain complex and birefringence images of the object via ptychographic phase retrieval. Using a camera with a pixel size of 3.45 μm, the method achieves birefringence imaging with a half-pitch resolution of 2.46 μm over a 59.74 mm2 field-of-view, which corresponds to a space-bandwidth product of 9.9 megapixels. We demonstrate the high-resolution, large-area, phase and birefringence imaging capability of our method by presenting the phase and birefringence images of various anisotropic objects, including a monosodium urate crystal, and excised mouse eye and heart tissues.
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1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Radiology (영상의학교실) > 1. Journal Papers
Yonsei Authors
Oh, Seung Jae(오승재)
Huh, Yong Min(허용민) ORCID logo https://orcid.org/0000-0002-9831-4475
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