0 93

Cited 0 times in

Enzyme-linked immunosorbent assay of IL-8 production in response to silver nanoparticles

Authors
 Eun-Jeong Yang  ;  Jiyoung Jang  ;  Dae-Hyoun Lim  ;  In-Hong Choi 
Citation
 Methods in Molecular Biology (Clifton, N.J.), Vol.926(131) : 139, 2012 
Journal Title
Methods in Molecular Biology (Clifton, N.J.)
ISSN
 1064-3745 
Issue Date
2012
MeSH
Enzyme-Linked Immunosorbent Assay / methods* ; Humans ; Interleukin-8 / biosynthesis* ; Metal Nanoparticles / toxicity* ; Reference Standards ; Silver / toxicity* ; Specimen Handling ; Spectrophotometry ; U937 Cells
Abstract
Enzyme-linked immunosorbent assay (ELISA) for monitoring the effects of nanoparticles on immune cells is a conventional method of assessing the levels of cytokine that are released into the culture supernatant after the addition of nanoparticles to a macrophage culture. However, it has been found that the presence of nanoparticles can interfere with spectrophotometric analysis, used as an indicator test system; thus, it is necessary to thoroughly checked for the possibility of interference. In this chapter, the assessment method of cytokine production is covered in detail by utilizing the cytokine model produced by silver nanoparticles.
Full Text
https://link.springer.com/protocol/10.1007/978-1-62703-002-1_9
DOI
10.1007/978-1-62703-002-1_9
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Yang, Eun Jeong(양은정) ORCID logo https://orcid.org/0000-0003-3516-1229
Choi, In Hong(최인홍) ORCID logo https://orcid.org/0000-0001-9851-0137
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/196652
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links