방광암세포주에서 IFN-γ의 효과

Abstract

The purpose of this study is to analyze the effects of IFN-r(interferon-r) on HLA-DR and ICAM-l(intercellular adhesion molecule 1) expression, a measure of cancer progression. We uses human bladder tumor cell lines of RT4(GI) and T24(GIII), a part of each type of cultured cells were treated by 200U/m IFN-r and divided into 3 groups( 24-hour, 48-hour, 72-hour) according to the time after the IFN-r treatment. Each cell was then indirectly immunofluorescence stained and analyzed using the Fluorescence Activated Cell Sorter(FACS). We founded that bladder cancer cells, when they are not treated by IFN-r, do not seem to show much HLA-DR constitutive expression. When bladder cancer cells are treated by IFN-r, the effects of IFN-r on HLA-DR expression rate seem to depend on the degree of differentiation of bladder cancer cells : well-differentiated bladder cancer cell shows higher HLA-DR expression rate when it is treated by IFN-r, while poorly-differentiated bladder cancer cell does not show much changes in HLA-DR expression rate even after it is treated by IFN-r. Bladder cancer cells of higher degree of malignancy show higher ICAM-1 constitutive expression rate and enhanced expression by IFN-r