Cited 7 times in
TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 노신혜 | - |
dc.contributor.author | 신동훈 | - |
dc.contributor.author | 이민구 | - |
dc.contributor.author | 이재면 | - |
dc.contributor.author | 최재영 | - |
dc.contributor.author | 강정민 | - |
dc.contributor.author | 박학 | - |
dc.contributor.author | 박필구 | - |
dc.date.accessioned | 2022-12-22T03:01:01Z | - |
dc.date.available | 2022-12-22T03:01:01Z | - |
dc.date.issued | 2022-08 | - |
dc.identifier.issn | * | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/191773 | - |
dc.description.abstract | Under ER stress conditions, the ER form of transmembrane proteins can reach the plasma membrane via a Golgi-independent unconventional protein secretion (UPS) pathway. However, the targeting mechanisms of membrane proteins for UPS are unknown. Here, this study reports that TMED proteins play a critical role in the ER stress-associated UPS of transmembrane proteins. The gene silencing results reveal that TMED2, TMED3, TMED9 and TMED10 are involved in the UPS of transmembrane proteins, such as CFTR, pendrin and SARS-CoV-2 Spike. Subsequent mechanistic analyses indicate that TMED3 recognizes the ER core-glycosylated protein cargos and that the heteromeric TMED2/3/9/10 complex mediates their UPS. Co-expression of all four TMEDs improves, while each single expression reduces, the UPS and ion transport function of trafficking-deficient ΔF508-CFTR and p.H723R-pendrin, which cause cystic fibrosis and Pendred syndrome, respectively. In contrast, TMED2/3/9/10 silencing reduces SARS-CoV-2 viral release. These results provide evidence for a common role of TMED3 and related TMEDs in the ER stress-associated, Golgi-independent secretion of transmembrane proteins. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.language | English | - |
dc.publisher | WILEY-VCH | - |
dc.relation.isPartOf | ADVANCED SCIENCE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.subject.MESH | COVID-19* / metabolism | - |
dc.subject.MESH | Cystic Fibrosis Transmembrane Conductance Regulator* / genetics | - |
dc.subject.MESH | Cystic Fibrosis Transmembrane Conductance Regulator* / metabolism | - |
dc.subject.MESH | Endoplasmic Reticulum Stress* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Protein Transport | - |
dc.subject.MESH | SARS-CoV-2 | - |
dc.subject.MESH | Spike Glycoprotein, Coronavirus* / metabolism | - |
dc.subject.MESH | Sulfate Transporters* / genetics | - |
dc.subject.MESH | Sulfate Transporters* / metabolism | - |
dc.subject.MESH | Vesicular Transport Proteins / metabolism | - |
dc.title | TMED3 Complex Mediates ER Stress-Associated Secretion of CFTR, Pendrin, and SARS-CoV-2 Spike | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | BioMedical Science Institute (의생명과학부) | - |
dc.contributor.googleauthor | Hak Park | - |
dc.contributor.googleauthor | Soo Kyung Seo | - |
dc.contributor.googleauthor | Ju-Ri Sim | - |
dc.contributor.googleauthor | Su Jin Hwang | - |
dc.contributor.googleauthor | Ye Jin Kim | - |
dc.contributor.googleauthor | Dong Hoon Shin | - |
dc.contributor.googleauthor | Dong Geon Jang | - |
dc.contributor.googleauthor | Shin Hye Noh | - |
dc.contributor.googleauthor | Pil-Gu Park | - |
dc.contributor.googleauthor | Si Hwan Ko | - |
dc.contributor.googleauthor | Mi Hwa Shin | - |
dc.contributor.googleauthor | Jae Young Choi | - |
dc.contributor.googleauthor | Yukishige Ito | - |
dc.contributor.googleauthor | Chung-Min Kang | - |
dc.contributor.googleauthor | Jae Myun Lee | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.identifier.doi | 10.1002/advs.202105320 | - |
dc.contributor.localId | A01285 | - |
dc.contributor.localId | A05644 | - |
dc.contributor.localId | A02781 | - |
dc.contributor.localId | A03071 | - |
dc.contributor.localId | A04173 | - |
dc.contributor.localId | A05078 | - |
dc.relation.journalcode | J04017 | - |
dc.identifier.eissn | 2198-3844 | - |
dc.identifier.pmid | 35748162 | - |
dc.subject.keyword | CFTR | - |
dc.subject.keyword | SARS-CoV-2 spike | - |
dc.subject.keyword | TMED | - |
dc.subject.keyword | UPS | - |
dc.subject.keyword | pendrin | - |
dc.contributor.alternativeName | Noh, Shin Hye | - |
dc.contributor.affiliatedAuthor | 노신혜 | - |
dc.contributor.affiliatedAuthor | 신동훈 | - |
dc.contributor.affiliatedAuthor | 이민구 | - |
dc.contributor.affiliatedAuthor | 이재면 | - |
dc.contributor.affiliatedAuthor | 최재영 | - |
dc.contributor.affiliatedAuthor | 강정민 | - |
dc.citation.volume | 9 | - |
dc.citation.number | 24 | - |
dc.citation.startPage | e2105320 | - |
dc.identifier.bibliographicCitation | ADVANCED SCIENCE, Vol.9(24) : e2105320, 2022-08 | - |
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