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Single-Cell RNA Sequencing Analysis of Human Dental Pulp Stem Cell and Human Periodontal Ligament Stem Cell

 Sukjoon Lee  ;  Dongzi Chen  ;  Munsu Park  ;  Sunil Kim  ;  Yoon Jeong Choi  ;  Seok Jun Moon  ;  Dong Min Shin  ;  Ji Hyun Lee  ;  Euiseong Kim 
 JOURNAL OF ENDODONTICS, Vol.48(2) : 240-248, 2022-02 
Journal Title
Issue Date
Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Dental Pulp* ; Humans ; Osteogenesis ; Periodontal Ligament* ; Sequence Analysis, RNA ; Stem Cells
gene expression analysis ; human dental pulp stem cell ; human periodontal ligament stem cell ; single-cell RNA sequencing
Introduction: Many studies have investigated the transcriptome profiles of dental stem cells for regenerative medicine. However, such studies use bulk RNA and do not consider cell-level heterogeneity. Here, we investigated the characteristics and heterogeneity of human dental pulp stem cells (hDPSCs) and human periodontal ligament stem cells (hPDLSCs) at the single-cell level and examined the differences between them.

Methods: hDPSCs and hPDLSCs were obtained from caries-free premolars (n = 2). Single-cell RNA sequencing of hDPSCs and hPDLSCs was performed using a Single Emulsion Systems (Dolomite Microfluidics, Royston, UK) droplet microfluidic device and the Illumina NextSeq550 system (Illumina, San Diego, CA). Data alignment was performed using STAR v2.4.0, and further analysis was performed using the Seurat, singleR, and clusterProfiler packages in R (R Foundation for Statistical Computing, Vienna, Austria).

Results: hDPSCs and hPDLSCs were separated into 3 clusters. hDPSCs mainly exhibited osteogenic and neurogenic cell populations. The main populations of hPDLSCs comprised osteogenic and myofibroblastic populations. hPDLSCs showed high "scores" for osteogenic gene expression, whereas hDPSCs had high neurogenic and endogenic scores.

Conclusions: Single-cell RNA sequencing of hDPSCs and hPDLSCs revealed that the genes were expressed in specific clusters. The results of these analyses can be used as reference databases and valuable resources for further research in dental therapeutics.
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2. College of Dentistry (치과대학) > Dept. of Conservative Dentistry (보존과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Sun Il(김선일) ORCID logo https://orcid.org/0000-0002-8889-9844
Kim, Eui Seong(김의성) ORCID logo https://orcid.org/0000-0003-2126-4761
Moon, Seok Jun(문석준) ORCID logo https://orcid.org/0000-0001-7282-2888
Shin, Dong Min(신동민) ORCID logo https://orcid.org/0000-0001-6042-0435
Lee, Sukjoon(이석준)
Choi, Yoon Jeong(최윤정) ORCID logo https://orcid.org/0000-0003-0781-8836
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