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General gene expression patterns and stemness of the gingiva and dental pulp

Authors
 Ko Eun Lee  ;  Chung-Min Kang  ;  Mijeong Jeon  ;  Seong-Oh Kim  ;  Jae-Ho Lee  ;  Hyung-Jun Choi 
Citation
 JOURNAL OF DENTAL SCIENCES, Vol.17(1) : 284-292, 2022-01 
Journal Title
JOURNAL OF DENTAL SCIENCES
ISSN
 1991-7902 
Issue Date
2022-01
Keywords
Dental pulp tissue ; Gene expression patterns ; Gingiva ; Inducible pluripotent stem cells ; Mesenchymal stem cells
Abstract
Background/purpose: Due to the unique properties of healing processes and cellular differentiation, the gingiva and dental pulp have attracted attention as a potential source of mesenchymal stem cells (MSCs). The purpose of this study was to obtain molecular-level information on these tissues in terms of their function and differentiation processes and investigate stemness.

Materials and methods: Healthy gingival tissues were collected from patients (n = 9; aged 7-12 years) who underwent simple surgical procedures, and normal dental pulp tissues were obtained from patients (n = 25; aged 11-25 years) undergoing tooth extraction for orthodontic reasons. Complementary DNA microarray, qRT-qPCR, and immunohistochemical staining were performed to assess general and MSC gene expression patterns.

Results: In the gingival tissue, genes related to keratinization, the formation of epithelial cells and ectoderm, and immune and/or inflammatory responses were highly expressed. Meanwhile, in the dental pulp tissue, genes related to ion transport, neuronal development and axon guidance, bone and enamel mineralization, extracellular matrix organization, and angiogenesis were highly expressed. When focusing on the expression of MSC genes, induced pluripotent stem (iPS) cell genes, such as Sox2, c-Myc, and KLF4, were expressed at higher levels in the gingival tissue, whereas dental stem cell genes, such as NT5E and VCAM1, were expressed in dental pulp tissue.

Conclusion: We found different general and MSC gene expression patterns between the gingival and dental pulp tissue. These results have implications for future regenerative medicine, considering the application of gingival tissue as a potential source of iPS cells.
Files in This Item:
T202200209.pdf Download
DOI
10.1016/j.jds.2021.02.012
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Chung Min(강정민) ORCID logo https://orcid.org/0000-0001-7813-3741
Kim, Seong Oh(김성오) ORCID logo https://orcid.org/0000-0002-8620-1377
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Choi, Hyung Jun(최형준) ORCID logo https://orcid.org/0000-0002-3315-6912
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187883
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