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Distinctive cytokine profiles of stem cells from human exfoliated deciduous teeth and dental pulp stem cells

Authors
 Chung-Min Kang  ;  Min Kyung Shin  ;  Mijeong Jeon  ;  Yong-Hyuk Lee  ;  Je Seon Song  ;  Jae-Ho Lee 
Citation
 JOURNAL OF DENTAL SCIENCES, Vol.17(1) : 276-283, 2022-01 
Journal Title
JOURNAL OF DENTAL SCIENCES
ISSN
 1991-7902 
Issue Date
2022-01
Keywords
Cytokine membrane array ; DPSCs ; Paracrine effect ; SHED ; Tissue regeneration
Abstract
Background/purpose: SHED and DPSC have stem cell regenerative potential, but comparative research on their cytokine profile is rare. This study aimed to investigate and compare cytokine profiles secreted from stem cells from human exfoliated deciduous teeth (SHED) and dental pulp stem cells (DPSCs).

Materials and methods: SHED-conditioned medium (CM) and DPSC-CM were extracted using seven primary and permanent teeth each. Cytokine membrane array was performed for each CM to quantify and compare the secretomes of 120 cytokines. Enzyme-linked immunosorbent assay, immunocytochemistry, and immunohistochemistry analysis were performed to demonstrate cytokine membrane array analysis.

Results: Significant differences were observed in the expression levels of 68 cytokines-27 and 41 cytokines were 1.3-fold more strongly expressed in SHED-CM and DPSC-CM, respectively. Cytokines involved in immunomodulation, odontogenesis and osteogenesis were more strongly expressed in SHED-CM. Cytokines involved in angiogenesis were detected more strongly in DPSCs-CM. SHED and DPSCs have distinctive cytokine profiles and characteristics in terms of their stem cell regenerative potential.

Conclusion: These observations suggest that SHED may have a better cytokine profile related to inflammatory, proliferative, osteogenic, and odontogenic potential.
Files in This Item:
T202200208.pdf Download
DOI
10.1016/j.jds.2021.03.019
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Pediatric Dentistry (소아치과학교실) > 1. Journal Papers
Yonsei Authors
Kang, Chung Min(강정민) ORCID logo https://orcid.org/0000-0001-7813-3741
Song, Je Seon(송제선) ORCID logo https://orcid.org/0000-0001-8620-5629
Lee, Jae Ho(이제호) ORCID logo https://orcid.org/0000-0002-1556-3485
Jeon, Mi Jeong(전미정)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187882
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