52 67

Cited 0 times in

BBS7-SHH Signaling Activity Regulates Primary Cilia for Periodontal Homeostasis

Authors
 Pi En Chang  ;  Shujin Li  ;  Hyun-Yi Kim  ;  Dong-Joon Lee  ;  Yoon Jeong Choi  ;  Han-Sung Jung 
Citation
 FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, Vol.9 : 796274, 2021-12 
Journal Title
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
Issue Date
2021-12
Keywords
BBS7 gene ; occlusal hypofunction ; periodontal homeostasis ; primary cilia ; shh signaling pathway
Abstract
Objectives: Mechanical stimuli are essential for the maintenance of periodontal ligament (PDL) homeostasis. Although there are several studies on atrophic changes in PDL due to occlusal hypofunction, the underlying mechanism is still unknown. Here, we aimed to explore the changes of gene expression in occlusal hypofunctional PDL and elucidate the related role in maintaining the PDL homeostasis. Methods: To investigate the transcriptomic difference between control and hypofunctional PDL tissue from patients, RNA sequencing was performed on 34 human teeth. The atrophic changes in PDL were evaluated by histological analysis. The effect of the Bardet-Biedl syndrome 7 (BBS7) knockdown was evaluated by the RT-qPCR, Western blot, wound healing, and tubule formation assay. Results: We detected that the expression of BBS7 was downregulated in occlusal hypofunctional PDL through RNA sequencing. Dynamic changes, including the number of periodontal ligament cells, alignment of collagen fibers, diameter of blood vessels, appearance of primary cilia, and torturous oxytalan fibers, were observed following occlusal hypofunction. Furthermore, Sonic hedgehog signaling (Shh) activity was closely associated with BBS7 expression in PDL cells. In addition, the cell migration and angiogenesis were also suppressed by BBS7 knockdown in vitro. Conclusion: We suggest that BBS7 plays an essential role in maintaining Shh signaling activity for PDL homeostasis.
Files in This Item:
T202105687.pdf Download
DOI
10.3389/fcell.2021.796274
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Oral Biology (구강생물학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Orthodontics (교정과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Lee, Dong Joon(이동준) ORCID logo https://orcid.org/0000-0001-6532-9729
Jung, Han Sung(정한성) ORCID logo https://orcid.org/0000-0003-2795-531X
Choi, Yoon Jeong(최윤정) ORCID logo https://orcid.org/0000-0003-0781-8836
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/187439
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links