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An Escherichia coli strain with extra catalase activity protects against murine colitis by scavenging hydrogen peroxide and regulating regulatory t cell/interleukin-17 pathways

Authors
 Da Hye Kim  ;  Jihye Park  ;  Soochan Kim  ;  Mi Young Yoon  ;  Hyun Woo Ma  ;  I Seul Park  ;  Mijeong Son  ;  Ji Hyung Kim  ;  Tae Il Kim  ;  Won Ho Kim  ;  Sang Sun Yoon  ;  Seung Won Kim  ;  Jae Hee Cheon 
Citation
 FREE RADICAL BIOLOGY AND MEDICINE, Vol.174 : 110-120, 2021-10 
Journal Title
FREE RADICAL BIOLOGY AND MEDICINE
ISSN
 0891-5849 
Issue Date
2021-10
MeSH
Animals ; Catalase ; Colitis* / chemically induced ; Colitis* / drug therapy ; Cytokines ; Dextran Sulfate / toxicity ; Disease Models, Animal ; Escherichia coli / genetics ; Hydrogen Peroxide ; Interleukin-17 ; Mice ; Mice, Knockout ; T-Lymphocytes, Regulatory*
Keywords
Hydrogen peroxide ; Inflammatory bowel disease ; Interleukin 17A ; Probiotics ; Regulatory T cell
Abstract
Background: Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the gastrointestinal tract whose occurrence is attributed to various factors, including genetic factors, immune response, microbial changes, and oxidative stress. Microbial-targeted therapy has emerged as an alternative to immunosuppressive therapy for IBD. Methods: The effects of an atypical commensal Escherichia coli strain harboring an additional catalase gene (compared to typical E. coli strain) on dextran sulfate sodium (DSS)-induced colitis were explored in mice. Results: The atypical E. coli (atEc) significantly restored body weight, reduced disease activity score, and improved histological scores in mice with colitis. Hydrogen peroxide levels in colitis mice were noticeably decreased when the mice were administered atEc. The proinflammatory cytokine levels were decreased and regulatory T cell numbers were increased after the administration of atEc. The abundance of Firmicutes was significantly recovered, while that of Proteobacteria decreased in atEc -treated mice compared with that in vehicle-treated wild-type mice. To investigate the role of interleukin (IL)-17A in mediating the anti-inflammatory effects of the atEc, IL-17A‒knockout mice were orally administered atEc. Clinical and immune responses and microbial composition were significantly reduced in IL-17A‒knockout mice compared with those in wild-type mice. Conclusions: atEc ameliorates colonic inflammation by controlling hydrogen peroxide levels, immune responses (including regulatory T cells and IL-17A), and microbial composition. atEc could be a novel candidate of probiotic for IBD treatment.
Full Text
https://www.sciencedirect.com/science/article/pii/S0891584921004421
DOI
10.1016/j.freeradbiomed.2021.08.002
Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Others (기타) > 1. Journal Papers
Yonsei Authors
Kim, Seung Won(김승원) ORCID logo https://orcid.org/0000-0002-1692-1192
Kim, Won Ho(김원호) ORCID logo https://orcid.org/0000-0002-5682-9972
Kim, Tae Il(김태일) ORCID logo https://orcid.org/0000-0003-4807-890X
Park, Ji Hye(박지혜)
Yoon, Mi Young(윤미영)
Yoon, Sang Sun(윤상선) ORCID logo https://orcid.org/0000-0003-2979-365X
Cheon, Jae Hee(천재희) ORCID logo https://orcid.org/0000-0002-2282-8904
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/185442
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